Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection
Joint Authors
Rosales-Encina, José Luis
Rodríguez-Morales, Olivia
Aranda-Fraustro, Alberto
Arce-Fonseca, Minerva
Carabarin-Lima, Alejandro
González-Vázquez, María Cristina
Graullera-Rivera, Verónica
Reyes, Pedro A.
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-07
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Trypanosoma cruzi is the protozoan parasite that causes Chagas disease, which is considered by the World Health Organization to be a neglected tropical disease.
Two drugs exist for the treatment of Chagas disease, nifurtimox and benznidazole; they are only effective in the acute phase, and a vaccine is currently not available.
In this study, we used the recombinant enolase from T.
cruzi H8 strain (MHOM/MX/1992/H8 Yucatán) (rTcENO) and its encoding DNA (pBKTcENO) to immunize mice and evaluate their protective effects in an experimental murine model of acute phase infection.
Our results showed that mice vaccinated with rTcENO or its encoding DNA were able to generate typical specific antibodies (IgG1, IgG2a, and IgG2b), suggesting that a mixed Th1/Th2 immune response was induced.
The parasite burden in the blood was reduced to 69.8% and 71% in mice vaccinated with rTcENO and pBKTcENO, respectively.
The group vaccinated with rTcENO achieved 75% survival, in contrast to the group vaccinated with pBKTcENO that showed no survival in comparison to the control groups.
Moreover, rTcENO immunization elevated the production of IFN-γ and IL-2 after the parasite challenge, suggesting that the Th1-type immune response was polarized.
These results indicated that rTcENO could be used as a vaccine against Chagas disease.
American Psychological Association (APA)
Arce-Fonseca, Minerva& González-Vázquez, María Cristina& Rodríguez-Morales, Olivia& Graullera-Rivera, Verónica& Aranda-Fraustro, Alberto& Reyes, Pedro A.…[et al.]. 2018. Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1193176
Modern Language Association (MLA)
Arce-Fonseca, Minerva…[et al.]. Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection. Journal of Immunology Research No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1193176
American Medical Association (AMA)
Arce-Fonseca, Minerva& González-Vázquez, María Cristina& Rodríguez-Morales, Olivia& Graullera-Rivera, Verónica& Aranda-Fraustro, Alberto& Reyes, Pedro A.…[et al.]. Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1193176
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193176