Mitochondrial-Targeted Antioxidant MitoQ Prevents E. coli Lipopolysaccharide-Induced Accumulation of Triacylglycerol and Lipid Droplets Biogenesis in Epithelial Cells

Joint Authors

Fock, Ekaterina
Bachteeva, Vera
Lavrova, Elena
Parnova, Rimma

Source

Journal of Lipids

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-09-02

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Medicine
Science

Abstract EN

The effect of bacterial lipopolysaccharide (LPS) on eukaryotic cell could be accompanied by a significant metabolic shift that includes accumulation of triacylglycerol (TAG) in lipid droplets (LD), ubiquitous organelles associated with fatty acid storage, energy regulation and demonstrated tight spatial and functional connections with mitochondria.

The impairment of mitochondrial activity under pathological stimuli has been shown to provoke TAG storage and LD biogenesis.

However the potential mechanisms that link mitochondrial disturbances and TAG accumulation are not completely understood.

We hypothesize that mitochondrial ROS (mROS) may play a role of a trigger leading to subsequent accumulation of intracellular TAG and LD in response to a bacterial stimulus.

Using isolated epithelial cells from the frog urinary bladder, we showed that LPS decreased fatty acids oxidation, enhanced TAG deposition, and promoted LD formation.

LPS treatment did not affect the mitochondrial membrane potential but increased cellular ROS production and led to impairment of mitochondrial function as revealed by decreased ATP production and a reduced maximal oxygen consumption rate (OCR) and OCR directed at ATP turnover.

The mitochondrial-targeted antioxidant MitoQ at a dose of 25 nM did not prevent LPS-induced alterations in cellular respiration, but, in contrast to nonmitochondrial antioxidant α-tocopherol, reduced the effect of LPS on the generation of ROS, restored the LPS-induced decline of fatty acids oxidation, and prevented accumulation of TAG and LD biogenesis.

The data obtained indicate the key signaling role of mROS in the lipid metabolic shift that occurs under the impact of a bacterial pathogen in epithelial cells.

American Psychological Association (APA)

Fock, Ekaterina& Bachteeva, Vera& Lavrova, Elena& Parnova, Rimma. 2018. Mitochondrial-Targeted Antioxidant MitoQ Prevents E. coli Lipopolysaccharide-Induced Accumulation of Triacylglycerol and Lipid Droplets Biogenesis in Epithelial Cells. Journal of Lipids،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1193519

Modern Language Association (MLA)

Fock, Ekaterina…[et al.]. Mitochondrial-Targeted Antioxidant MitoQ Prevents E. coli Lipopolysaccharide-Induced Accumulation of Triacylglycerol and Lipid Droplets Biogenesis in Epithelial Cells. Journal of Lipids No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1193519

American Medical Association (AMA)

Fock, Ekaterina& Bachteeva, Vera& Lavrova, Elena& Parnova, Rimma. Mitochondrial-Targeted Antioxidant MitoQ Prevents E. coli Lipopolysaccharide-Induced Accumulation of Triacylglycerol and Lipid Droplets Biogenesis in Epithelial Cells. Journal of Lipids. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1193519

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1193519