Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism
Joint Authors
Zhang, Xiaoyu
Hou, Cui-Lan
Li, Wenguang
Li, Zengyou
Gao, Jing
Chen, Zhenjie
Zhao, Xiqiong
Yang, Yaya
Song, Yong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Isoliquiritigenin (ISL), a natural antioxidant, has antitumor activity in different types of cancer cells.
However the antitumor effect of ISL on human tongue squamous carcinoma cells (TSCC) is not clear.
Here we aimed to investigate the effects of synthetic isoliquiritigenin (S-ISL) on TSCC and elucidate the underlying mechanisms.
S-ISL was synthesized and elucidated from its nuclear magnetic resonance spectrum and examined using high performance liquid chromatography.
The effects of S-ISL on TSCC cells (Tca8113) were evaluated in relation to cell proliferation, apoptosis and adhesion, migration, and invasion using sulforhodamine B assay, fluorescence microscopy technique, flow cytometry (FCM) analysis, and Boyden chamber assay.
The associated regulatory mechanisms were examined using FCM and fluorescence microscopy for intracellular reactive oxygen species (ROS) generation, Gelatin zymography assay for matrix metalloproteinase (MMP) activities, and Western blot for apoptosis regulatory proteins (Bcl-2 and Bax).
Our data indicated that S-ISL inhibited Tca8113 cell proliferation, adhesion, migration, and invasion while promoting the cell apoptosis.
Such effects were accompanied by downregulation of Bcl-2 and upregulation of Bax, reduction of MMP-2 and MMP-9 activities, and decreased ROS production.
We conclude that S-ISL is a promising agent targeting TSCC through multiple anticancer effects, regulated by its antioxidant mechanism.
American Psychological Association (APA)
Hou, Cui-Lan& Li, Wenguang& Li, Zengyou& Gao, Jing& Chen, Zhenjie& Zhao, Xiqiong…[et al.]. 2017. Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1193799
Modern Language Association (MLA)
Hou, Cui-Lan…[et al.]. Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1193799
American Medical Association (AMA)
Hou, Cui-Lan& Li, Wenguang& Li, Zengyou& Gao, Jing& Chen, Zhenjie& Zhao, Xiqiong…[et al.]. Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1193799
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193799