Efficient Delivery of Therapeutic siRNA with Nanoparticles Induces Apoptosis in Prostate Cancer Cells
Joint Authors
Mu, Xupeng
Lu, Haibin
Fan, Lianlian
Yan, Shaohua
Hu, Kebang
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Gene silencing using small interfering RNA (siRNA) has shown significant potential in the treatment of cancer.
Herein, we developed the lipid-polymer hybrid nanoparticles (PEG-LP/siRNA NPs) for siRNA delivery.
The cell viability assay indicated that PEG-LP/siRNA NPs had negligible cell cytotoxicity.
The cellular uptake efficiency of PEG-LP/siRNA NPs measured by flow cytometry was up to 94.4%.
Importantly, in vitro gene knockdown experiments demonstrated that PEG-LP/siJnk-1 NPs could significantly downregulate the expression of Jnk-1 at both the mRNA and protein levels in DU145 cells.
Gene knockdown of Jnk-1 could activate apoptosis in part by the mitochondrial pathway in DU145 cells.
Moreover, the PEG-LP/siJnk-1 NPs could inhibit tumor growth in a DU145 xenograft murine model, suggesting its therapeutic promise in cancer therapy.
American Psychological Association (APA)
Mu, Xupeng& Lu, Haibin& Fan, Lianlian& Yan, Shaohua& Hu, Kebang. 2018. Efficient Delivery of Therapeutic siRNA with Nanoparticles Induces Apoptosis in Prostate Cancer Cells. Journal of Nanomaterials،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1194168
Modern Language Association (MLA)
Mu, Xupeng…[et al.]. Efficient Delivery of Therapeutic siRNA with Nanoparticles Induces Apoptosis in Prostate Cancer Cells. Journal of Nanomaterials No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1194168
American Medical Association (AMA)
Mu, Xupeng& Lu, Haibin& Fan, Lianlian& Yan, Shaohua& Hu, Kebang. Efficient Delivery of Therapeutic siRNA with Nanoparticles Induces Apoptosis in Prostate Cancer Cells. Journal of Nanomaterials. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1194168
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194168
