Blockage of NOX2MAPKNF-κB Pathway Protects Photoreceptors against Glucose Deprivation-Induced Cell Death
Joint Authors
Fan, Bin
Wang, Bei-Fen
Che, Lin
Sun, Ying-Jian
Liu, Shu-Yan
Li, Guang-Yu
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-11
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Acute energy failure is one of the critical factors contributing to the pathogenic mechanisms of retinal ischemia.
Our previous study demonstrated that glucose deprivation can lead to a caspase-dependent cell death of photoreceptors.
The aim of this study was to decipher the upstream signal pathway in glucose deprivation- (GD-) induced cell death.
We mimicked acute energy failure by using glucose deprivation in photoreceptor cells (661W cells).
GD-induced oxidative stress was evaluated by measuring ROS with the DCFH-DA assay and HO-1 expression by Western blot analysis.
The activation of NOX2/MAPK/NF-κB signal was assessed by Western blot and immunohistochemical assays.
The roles of these signals in GD-induced cell death were measured by using their specific inhibitors.
Inhibition of Rac-1 and NOX2 suppressed GD-induced oxidative stress and protected photoreceptors against GD-induced cell death.
NOX2 was an upstream signal in the caspase-dependent cell death cascade, yet the downstream MAPK pathways were activated and blocking MAPK signals rescued 661W cells from GD-induced death.
In addition, GD caused the activation of NF-κB signal and inhibiting NF-κB significantly protected 661W cells.
These observations may provide insights for treating retinal ischemic diseases and protecting retinal neurons from ischemia-induced cell death.
American Psychological Association (APA)
Fan, Bin& Wang, Bei-Fen& Che, Lin& Sun, Ying-Jian& Liu, Shu-Yan& Li, Guang-Yu. 2017. Blockage of NOX2MAPKNF-κB Pathway Protects Photoreceptors against Glucose Deprivation-Induced Cell Death. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1194868
Modern Language Association (MLA)
Fan, Bin…[et al.]. Blockage of NOX2MAPKNF-κB Pathway Protects Photoreceptors against Glucose Deprivation-Induced Cell Death. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1194868
American Medical Association (AMA)
Fan, Bin& Wang, Bei-Fen& Che, Lin& Sun, Ying-Jian& Liu, Shu-Yan& Li, Guang-Yu. Blockage of NOX2MAPKNF-κB Pathway Protects Photoreceptors against Glucose Deprivation-Induced Cell Death. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1194868
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194868