Quercetin and Quercetin-Rich Red Onion Extract Alter Pgc-1α Promoter Methylation and Splice Variant Expression
Joint Authors
Devarshi, Prasad P.
Jones, Aarin D.
Taylor, Erin M.
Stefanska, Barbara
Henagan, Tara M.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Pgc-1α and its various isoforms may play a role in determining skeletal muscle mitochondrial adaptations in response to diet.
8 wks of dietary supplementation with the flavonoid quercetin (Q) or red onion extract (ROE) in a high fat diet (HFD) ameliorates HFD-induced obesity and insulin resistance in C57BL/J mice while upregulating Pgc-1α and increasing skeletal muscle mitochondrial number and function.
Here, mice were fed a low fat (LF), high fat (HF), high fat plus quercetin (HF + Q), or high fat plus red onion extract (HF + RO) diet for 9 wks and skeletal muscle Pgc-1α isoform expression and DNA methylation were determined.
Quantification of various Pgc-1α isoforms, including isoforms Pgc-1α-a, Pgc-1α-b, Pgc-1α-c, Pgc-1α4, total NT-Pgc-1α, and FL-Pgc-1α, showed that only total NT-Pgc-1α expression was increased in LF, HF + Q, and HF + RO compared to HF.
Furthermore, Q supplementation decreased Pgc-1α-a expression compared to LF and HF, and ROE decreased Pgc-1α-a expression compared to LF.
FL-Pgc-1α was decreased in HF + Q and HF + RO compared to LF and HF.
HF exhibited hypermethylation at the −260 nucleotide (nt) in the Pgc-1α promoter.
Q and ROE prevented HFD-induced hypermethylation.
−260 nt methylation levels were associated with NT-Pgc-1α expression only.
Pgc-1α isoform expression may be epigenetically regulated by Q and ROE through DNA methylation.
American Psychological Association (APA)
Devarshi, Prasad P.& Jones, Aarin D.& Taylor, Erin M.& Stefanska, Barbara& Henagan, Tara M.. 2017. Quercetin and Quercetin-Rich Red Onion Extract Alter Pgc-1α Promoter Methylation and Splice Variant Expression. PPAR Research،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1197216
Modern Language Association (MLA)
Devarshi, Prasad P.…[et al.]. Quercetin and Quercetin-Rich Red Onion Extract Alter Pgc-1α Promoter Methylation and Splice Variant Expression. PPAR Research No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1197216
American Medical Association (AMA)
Devarshi, Prasad P.& Jones, Aarin D.& Taylor, Erin M.& Stefanska, Barbara& Henagan, Tara M.. Quercetin and Quercetin-Rich Red Onion Extract Alter Pgc-1α Promoter Methylation and Splice Variant Expression. PPAR Research. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1197216
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197216