Effects of Tumor Necrosis Factor-α on Morphology and Mechanical Properties of HCT116 Human Colon Cancer Cells Investigated by Atomic Force Microscopy
Joint Authors
Liu, Huiqing
Wang, Nan
Zhang, Zhe
Wang, Hongda
Tang, Jilin
Du, Jun
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Information Technology and Computer Science
Abstract EN
Chronic inflammation orchestrates the tumor microenvironment and is strongly associated with cancer.
Tumor necrosis factor-α (TNFα) is involved in tumor invasion and metastasis by inducing epithelial to mesenchymal transition (EMT).
This process is defined by the loss of epithelial characteristics and gain of mesenchymal traits.
The mechanisms of TNFα-induced EMT in cancer cells have been well studied.
However, mechanical properties have not yet been probed.
In this work, atomic force microscopy (AFM) was applied to investigate the morphology and mechanical properties of EMT in HCT116 human colon cancer cells.
A remarkable morphological change from cobblestone shape to spindle-like morphology was observed.
In parallel, AFM images showed that the cellular cytoskeleton was rearranged from a cortical to a stress-fiber pattern.
Moreover, cell stiffness measurements indicated that Young’s modulus of cells gradually reduced from 1 to 3 days with TNFα-treatment, but it has an apparent increase after 4 days of treatment compared with that for 3 days.
Additionally, Young’s modulus of the cells treated with TNFα for 4 days is slightly larger than that for 1 or 2 days, but still less than that of the untreated cells.
Our work contributes to a better understanding of colorectal cancer metastasis induced by inflammation.
American Psychological Association (APA)
Liu, Huiqing& Wang, Nan& Zhang, Zhe& Wang, Hongda& Du, Jun& Tang, Jilin. 2017. Effects of Tumor Necrosis Factor-α on Morphology and Mechanical Properties of HCT116 Human Colon Cancer Cells Investigated by Atomic Force Microscopy. Scanning،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1197839
Modern Language Association (MLA)
Liu, Huiqing…[et al.]. Effects of Tumor Necrosis Factor-α on Morphology and Mechanical Properties of HCT116 Human Colon Cancer Cells Investigated by Atomic Force Microscopy. Scanning No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1197839
American Medical Association (AMA)
Liu, Huiqing& Wang, Nan& Zhang, Zhe& Wang, Hongda& Du, Jun& Tang, Jilin. Effects of Tumor Necrosis Factor-α on Morphology and Mechanical Properties of HCT116 Human Colon Cancer Cells Investigated by Atomic Force Microscopy. Scanning. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1197839
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197839