Calycosin-7-O-β-D-glucoside Attenuates OGDR-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1FOXO1PGC-1α Pathway in HT22 Cells

Abstract EN

Neuronal apoptosis induced by oxidative stress is a major pathological process that occurs after cerebral ischemia-reperfusion.

Calycosin-7-O-β-D-glucoside (CG) is a representative component of isoflavones in Radix Astragali (RA).

Previous studies have shown that CG has potential neuroprotective effects.

However, whether CG alleviates neuronal apoptosis through antioxidant stress after ischemia-reperfusion remains unknown.

To investigate the positive effects of CG on oxidative stress and apoptosis of neurons, we simulated the ischemia-reperfusion process in vitro using an immortalized hippocampal neuron cell line (HT22) and oxygen-glucose deprivation/reperfusion (OGD/R) model.

CG significantly improved cell viability and reduced oxidative stress and neuronal apoptosis.

In addition, CG treatment upregulated the expression of SIRT1, FOXO1, PGC-1α, and Bcl-2 and downregulated the expression of Bax.

In summary, our findings indicate that CG alleviates OGD/R-induced damage via the SIRT1/FOXO1/PGC-1α signaling pathway.

Thus, CG maybe a promising therapeutic candidate for brain injury associated with ischemic stroke.