![](/images/graphics-bg.png)
Evaluating Wharton’s Jelly-Derived Mesenchymal Stem Cell’s Survival, Migration, and Expression of Wound Repair Markers under Conditions of Ischemia-Like Stress
Joint Authors
Himal, Iris
Goyal, Umesh
Ta, Malancha
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-07
Country of Publication
Egypt
No. of Pages
13
Abstract EN
The efficacy of mesenchymal stem cell (MSC) therapy is currently limited by low retention and poor survival of transplanted cells as demonstrated by clinical studies.
This is mainly due to the harsh microenvironment created by oxygen and nutrient deprivation and inflammation at the injured sites.
The choice of MSC source could be critical in determining fate and cellular function of MSCs under stress.
Our objective here was to investigate the influence of ischemia-like stress on Wharton’s jelly MSCs (WJ-MSCs) from human umbilical cord to assess their therapeutic relevance in ischemic diseases.
We simulated conditions of ischemia in vitro by culturing WJ-MSCs in 2% oxygen in serum deprived and low glucose medium.
Under these conditions, WJ-MSCs retained viable population of greater than 80%.
They expressed the characteristic MSC surface antigens at levels comparable to the control WJ-MSCs and were negative for the expression of costimulatory molecules.
An upregulation of many ECM and adhesion molecules and growth and angiogenic factors contributing to wound healing and regeneration was noted in the ischemic WJ-MSC population by a PCR array.
Their migration ability, however, got impaired.
Our findings provide evidence that WJ-MSCs might be therapeutically beneficial and potent in healing wounds under ischemic conditions.
American Psychological Association (APA)
Himal, Iris& Goyal, Umesh& Ta, Malancha. 2017. Evaluating Wharton’s Jelly-Derived Mesenchymal Stem Cell’s Survival, Migration, and Expression of Wound Repair Markers under Conditions of Ischemia-Like Stress. Stem Cells International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1201754
Modern Language Association (MLA)
Himal, Iris…[et al.]. Evaluating Wharton’s Jelly-Derived Mesenchymal Stem Cell’s Survival, Migration, and Expression of Wound Repair Markers under Conditions of Ischemia-Like Stress. Stem Cells International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1201754
American Medical Association (AMA)
Himal, Iris& Goyal, Umesh& Ta, Malancha. Evaluating Wharton’s Jelly-Derived Mesenchymal Stem Cell’s Survival, Migration, and Expression of Wound Repair Markers under Conditions of Ischemia-Like Stress. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1201754
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201754