Cytokine, Chemokine, and Growth Factor Profile Characterization of Undifferentiated and Osteoinduced Human Adipose-Derived Stem Cells
Joint Authors
Mussano, Federico
Carossa, Stefano
Genova, T.
Corsalini, M.
Pettini, F.
Di Venere, D.
Schierano, Gianmario
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-10
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Bone is the second most manipulated tissue after blood.
Adipose-derived stem cells (ASCs) may become a convenient source of MSC for bone regenerative protocols.
Surprisingly, little is known about the most significant biomolecules these cells produce and release after being osteoinduced.
Therefore, the present study aimed at dosing 13 candidates chosen among the most representative cytokines, chemokines, and growth factors within the conditioned media of osteodifferentiated and undifferentiated ASCs.
Two acknowledged osteoblastic cell models, that is, MG-63 and SaOs-2 cells, were compared.
Notably, IL-6, IL-8, MCP-1, and VEGF were highly produced and detectable in ASCs.
In addition, while IL-6 and IL-8 seemed to be significantly induced by the osteogenic medium, no such effect was seen for MCP-1 and VEGF.
Overall SaOS-2 had a poor expression profile, which may be consistent with the more differentiated phenotype of SaOs-2 compared to ASCs and MG-63.
Instead, in maintaining medium, MG-63 displayed a very rich production of IL-12, MCP-1, IP-10, and VEGF, which were significantly reduced in osteogenic conditions, with the only exception of MCP-1.
The high expression of MCP-1 and VEGF, even after the osteogenic commitment, may support the usage of ASCs in bone regenerative protocols by recruiting both osteoblasts and osteoclasts of the host.
American Psychological Association (APA)
Mussano, Federico& Genova, T.& Corsalini, M.& Schierano, Gianmario& Pettini, F.& Di Venere, D.…[et al.]. 2017. Cytokine, Chemokine, and Growth Factor Profile Characterization of Undifferentiated and Osteoinduced Human Adipose-Derived Stem Cells. Stem Cells International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1201955
Modern Language Association (MLA)
Mussano, Federico…[et al.]. Cytokine, Chemokine, and Growth Factor Profile Characterization of Undifferentiated and Osteoinduced Human Adipose-Derived Stem Cells. Stem Cells International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1201955
American Medical Association (AMA)
Mussano, Federico& Genova, T.& Corsalini, M.& Schierano, Gianmario& Pettini, F.& Di Venere, D.…[et al.]. Cytokine, Chemokine, and Growth Factor Profile Characterization of Undifferentiated and Osteoinduced Human Adipose-Derived Stem Cells. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1201955
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201955