Thymosin β4 Improves Differentiation and Vascularization of EHTs
Joint Authors
Ziegler, Tilman
Hinkel, Rabea
Stöhr, Andrea
Eschenhagen, Thomas
Laugwitz, Karl-Ludwig
le Noble, Ferdinand
David, Robert
Hansen, Arne
Kupatt, Christian
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-16
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Induced pluripotent stem cells (iPSC) constitute a powerful tool to study cardiac physiology and represents a promising treatment strategy to tackle cardiac disease.
However, iPSCs remain relatively immature after differentiation.
Additionally, engineered heart tissue (EHT) has been investigated as a therapy option in preclinical disease models with promising results, although their vascularization and functionality leave room for improvement.
Thymosin β4 (Tβ4) has been shown to promote the differentiation of progenitor cell lines to cardiomyocytes while it also induces angiogenic sprouting and vascular maturation.
We examined the potential impact of Tβ4 to enhance maturation of cardiomyocytes from iPSCs.
Assessing the expression of transcription factors associated with cardiac differentiation, we were able to demonstrate the increased generation of cells displaying cardiomyocyte characteristics in vitro.
Furthermore, we demonstrated, in a zebrafish model of embryonic vascular development, that Tβ4 is crucial for the proper execution of lymphatic and angiogenic vessel sprouting.
Finally, utilizing Tβ4-transduced EHTs generated from mice genetically engineered to label endothelial cells in vitro, we show that treatment with Tβ4 promotes vascularization and contractility in EHTs, highlighting Tβ4 as a growth factor improving the formation of cardiomyocytes from iPSC and enhancing the performance of EHTs generated from neonatal cardiomyocytes.
American Psychological Association (APA)
Ziegler, Tilman& Hinkel, Rabea& Stöhr, Andrea& Eschenhagen, Thomas& Laugwitz, Karl-Ludwig& le Noble, Ferdinand…[et al.]. 2017. Thymosin β4 Improves Differentiation and Vascularization of EHTs. Stem Cells International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1201989
Modern Language Association (MLA)
Ziegler, Tilman…[et al.]. Thymosin β4 Improves Differentiation and Vascularization of EHTs. Stem Cells International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1201989
American Medical Association (AMA)
Ziegler, Tilman& Hinkel, Rabea& Stöhr, Andrea& Eschenhagen, Thomas& Laugwitz, Karl-Ludwig& le Noble, Ferdinand…[et al.]. Thymosin β4 Improves Differentiation and Vascularization of EHTs. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1201989
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201989