Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-FreeSerum-Free Conditions
Joint Authors
Patrikoski, Mimmi
Lee, Michelle Hui Ching
Mäkinen, Laura
Ang, Xiu Min
Mannerström, Bettina
Raghunath, Michael
Miettinen, Susanna
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-30
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Microenvironment plays an important role for stem cell proliferation and differentiation.
Macromolecular crowding (MMC) was recently shown to assist stem cells in forming their own matrix microenvironment in vitro.
The ability of MMC to support adipose stem cell (ASC) proliferation, metabolism, and multilineage differentiation was studied under different conditions: fetal bovine serum- (FBS-) and human serum- (HS-) based media and xeno- and serum-free (XF/SF) media.
Furthermore, the immunophenotype of ASCs under MMC was evaluated.
The proliferative capacity of ASCs under MMC was attenuated in each condition.
However, osteogenic differentiation was enhanced under MMC, shown by increased deposition of mineralized matrix in FBS and HS cultures.
Likewise, significantly greater lipid droplet accumulation and increased collagen IV deposition indicated enhanced adipogenesis under MMC in FBS and HS cultures.
In contrast, chondrogenic differentiation was attenuated in ASCs expanded under MMC.
The ASC immunophenotype was maintained under MMC with significantly higher expression of CD54.
However, MMC impaired metabolic activity and differentiation capacity of ASCs in XF/SF conditions.
Both the supportive and inhibitory effects of MMC on ASC are culture condition dependent.
In the presence of serum, MMC maintains ASC immunophenotype and enhances adipogenic and osteogenic differentiation at the cost of reduced proliferation.
American Psychological Association (APA)
Patrikoski, Mimmi& Lee, Michelle Hui Ching& Mäkinen, Laura& Ang, Xiu Min& Mannerström, Bettina& Raghunath, Michael…[et al.]. 2017. Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-FreeSerum-Free Conditions. Stem Cells International،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1201993
Modern Language Association (MLA)
Patrikoski, Mimmi…[et al.]. Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-FreeSerum-Free Conditions. Stem Cells International No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1201993
American Medical Association (AMA)
Patrikoski, Mimmi& Lee, Michelle Hui Ching& Mäkinen, Laura& Ang, Xiu Min& Mannerström, Bettina& Raghunath, Michael…[et al.]. Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-FreeSerum-Free Conditions. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1201993
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201993