Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell Therapy
Joint Authors
Goldberg, Anna Carla
Paladino, Fernanda Vieira
Piccinato, Carla Azevedo
Sardinha, Luiz Roberto
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-05
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Wharton’s jelly mesenchymal stem cells (WJ-MSC) exhibit immunomodulatory effects on T cell response.
WJ-MSC are easy to collect, process, and proliferate rapidly in culture, but information on the variability of individual cell samples impacting upon in vitro expansion, immunomodulatory potential, and aging processes is still lacking.
We propose to evaluate the immunomodulatory cytokine profile and capacity to inhibit T cell proliferation of WJ-MSC progressing to replicative senescence in order to analyze if expected responses are affected.
Our results show that the gene expression of immunomodulatory molecules varied among samples with no specific pattern present.
In coculture, all WJ-MSC were capable of inhibiting mitogen-activated CD3+ T cell proliferation, although to different degrees, and each PBMC responded with a different level of inhibition.
Thus, we suggest that each WJ-MSC displays unique behavior, differing in patterns of cytokine mRNA expression and immunomodulatory capacity.
We believe that variability between samples may play a role in the effectiveness of WJ-MSC employed therapeutically.
American Psychological Association (APA)
Paladino, Fernanda Vieira& Sardinha, Luiz Roberto& Piccinato, Carla Azevedo& Goldberg, Anna Carla. 2017. Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell Therapy. Stem Cells International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1202270
Modern Language Association (MLA)
Paladino, Fernanda Vieira…[et al.]. Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell Therapy. Stem Cells International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1202270
American Medical Association (AMA)
Paladino, Fernanda Vieira& Sardinha, Luiz Roberto& Piccinato, Carla Azevedo& Goldberg, Anna Carla. Intrinsic Variability Present in Wharton’s Jelly Mesenchymal Stem Cells and T Cell Responses May Impact Cell Therapy. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1202270
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202270