Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPRCas9
Joint Authors
Koizumi, Noriko
Higashioka, Koki
Sakurai, Hidetoshi
Sato, Takahiko
Sotozono, Chie
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-04
Country of Publication
Egypt
No. of Pages
9
Abstract EN
It is well known that myogenic regulatory factors encoded by the Myod1 family of genes have pivotal roles in myogenesis, with partially overlapping functions, as demonstrated for the mouse embryo.
Myogenin-mutant mice, however, exhibit severe myogenic defects without compensation by other myogenic factors.
MYOGENIN might be expected to have an analogous function in human myogenic cells.
To verify this hypothesis, we generated MYOGENIN-mutated human iPS cells by using CRISPR/Cas9 genome-editing technology.
Our results suggest that MYOD1-independent or MYOD1-dependent mechanisms can compensate for the loss of MYOGENIN and that these mechanisms are likely to be crucial for regulating skeletal muscle differentiation and formation.
American Psychological Association (APA)
Higashioka, Koki& Koizumi, Noriko& Sakurai, Hidetoshi& Sotozono, Chie& Sato, Takahiko. 2017. Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPRCas9. Stem Cells International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1202342
Modern Language Association (MLA)
Higashioka, Koki…[et al.]. Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPRCas9. Stem Cells International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1202342
American Medical Association (AMA)
Higashioka, Koki& Koizumi, Noriko& Sakurai, Hidetoshi& Sotozono, Chie& Sato, Takahiko. Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPRCas9. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1202342
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202342