5α,6α-Epoxyphytosterols and 5α,6α-Epoxycholesterol Increase Oxidative Stress in Rats on Low-Cholesterol Diet
Joint Authors
Owczarek, Aleksander
Stanek, Agata
Cholewka, Armand
Wielkoszyński, Tomasz
Strzelczyk, Joanna Katarzyna
Zalejska-Fiolka, Jolanta
Krawczyk, Aneta
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
Cholesterol oxidation products have an established proatherogenic and cytotoxic effect.
An increased exposure to these substances may be associated with the development of atherosclerosis and cancers.
Relatively little, though, is known about the effect of phytosterol oxidation products, although phytosterols are present in commonly available and industrial food products.
Thus, the aim of the research was to assess the effect of 5α,6α-epoxyphytosterols, which are important phytosterol oxidation products, on redox state in rats.
Material and Methods.
The animals were divided into 3 groups and exposed to nutritional sterols by receiving feed containing 5α,6α-epoxyphytosterols (ES group) and 5α,6α-epoxycholesterol (Ech group) or sterol-free feed (C group).
The levels of malondialdehyde (MDA), conjugated dienes (CD), and ferric reducing antioxidant potential (FRAP) were assayed in the plasma; anti-7-ketocholesterol antibodies and activity of paraoxonase-1 (PON1) were determined in serum, whereas the activity of catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), S-glutathione transferase (GST), and superoxide dismutase (SOD) were assayed in RBCs.
Results.
During the experiment, the levels of lipid peroxidation products increased, such as CD and anti-7-ketocholesterol antibodies.
At the same time, the plasma levels of FRAP and serum activity of PON1 decreased alongside the reduced activity of GPx, GR, and SOD in RBCs.
There was no effect of the studied compounds on the plasma MDA levels or on the activity of CAT and GST in RBCs.
Conclusions.
Both 5α,6α-epoxyphytosterols and 5α,6α-epoxycholesterols similarly dysregulate the redox state in experimental animal model and may significantly impact atherogenesis.
American Psychological Association (APA)
Wielkoszyński, Tomasz& Zalejska-Fiolka, Jolanta& Strzelczyk, Joanna Katarzyna& Owczarek, Aleksander& Cholewka, Armand& Krawczyk, Aneta…[et al.]. 2019. 5α,6α-Epoxyphytosterols and 5α,6α-Epoxycholesterol Increase Oxidative Stress in Rats on Low-Cholesterol Diet. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1202466
Modern Language Association (MLA)
Wielkoszyński, Tomasz…[et al.]. 5α,6α-Epoxyphytosterols and 5α,6α-Epoxycholesterol Increase Oxidative Stress in Rats on Low-Cholesterol Diet. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1202466
American Medical Association (AMA)
Wielkoszyński, Tomasz& Zalejska-Fiolka, Jolanta& Strzelczyk, Joanna Katarzyna& Owczarek, Aleksander& Cholewka, Armand& Krawczyk, Aneta…[et al.]. 5α,6α-Epoxyphytosterols and 5α,6α-Epoxycholesterol Increase Oxidative Stress in Rats on Low-Cholesterol Diet. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1202466
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202466