An Enriched Environment Enhances Angiogenesis Surrounding the Cingulum in Ischaemic Stroke Rats
Joint Authors
Xing, Ying
Yu, Kewei
Liu, Gang
Xie, Hongyu
Wu, Yi
Wang, Fei
Shen, Xueyan
Luo, Lu
Tian, Shan
Bao, Chunrong
Fan, Yunhui
Wang, Nianhong
Zhang, Qun
Liu, Li
Li, Siyue
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-12
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
An enriched environment (EE) has been demonstrated to improve functional recovery in animal models of ischaemic stroke through enhancing vascular endothelial growth factor- (VEGF-) mediated neuroprotection accompanied by angiogenesis in the ischaemic hemisphere.
Whether EEs also promote VEGF-mediated neuroprotection and angiogenesis in the contralateral hemisphere remains unclear.
Here, we explored the effect of EEs on VEGF expression and angiogenesis within the contralateral cerebral cortex in a rat middle cerebral artery occlusion/reperfusion (MCAO/r) model.
We assessed the expression levels of platelet endothelial cell adhesion molecule-1 (CD31), VEGF, and endothelial nitric oxide synthase (eNOS) in the whole contralateral cerebral cortex using Western blotting assay but did not find an increase in the expression of CD31, VEGF, or eNOS in MCAO/r rats housed in EEs, which suggested that EEs did not enhance the overall expression of VEGF and eNOS or angiogenesis in the entire contralateral cortex.
We further analysed the local effect of EEs by immunohistochemistry and found that in and around the bilateral cingulum in MCAO/r rats housed in EEs, haematopoietic progenitor cell antigen- (CD34-) positive endothelial progenitor cells were significantly increased compared with those of rats housed in standard cages (SCs).
Further experiments showed that EEs increased neuronal VEGF expression surrounding the cingulum in MCAO/r rats and robustly upregulated eNOS expression.
These results revealed that EEs enhanced angiogenesis, VEGF expression, and activation of the VEGF-eNOS pathway in and/or around the cingulum in MCAO/r rats, which were involved in the functional recovery of MCAO/r rats.
American Psychological Association (APA)
Shen, Xueyan& Luo, Lu& Wang, Fei& Yu, Kewei& Xie, Hongyu& Tian, Shan…[et al.]. 2020. An Enriched Environment Enhances Angiogenesis Surrounding the Cingulum in Ischaemic Stroke Rats. Neural Plasticity،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1202891
Modern Language Association (MLA)
Shen, Xueyan…[et al.]. An Enriched Environment Enhances Angiogenesis Surrounding the Cingulum in Ischaemic Stroke Rats. Neural Plasticity No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1202891
American Medical Association (AMA)
Shen, Xueyan& Luo, Lu& Wang, Fei& Yu, Kewei& Xie, Hongyu& Tian, Shan…[et al.]. An Enriched Environment Enhances Angiogenesis Surrounding the Cingulum in Ischaemic Stroke Rats. Neural Plasticity. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1202891
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202891