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Reduced NLRP3 Gene Expression Limits the IL-1β Cleavage via Inflammasome in Monocytes from Severely Injured Trauma Patients
Joint Authors
Sturm, Ramona
Kany, Shinwan
Horstmann, Johann-Philipp
Mörs, Katharina
Relja, Borna
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
Traumatic injury or severe surgery leads to a profound immune response with a diminished functionality of monocytes and subsequently their IL-1β release.
IL-1β plays an important role in host immunity and protection against infections.
Its biological activation via IL-1β-precursor processing requires the transcription of inflammasome components and their activation.
Deregulated activity of NOD-like receptor inflammasomes (NLR) like NLRP3 that leads to the maturation of IL-1β has been described in various diseases.
While the role of other inflammasomes has been studied in monocytes, nothing is known about NLRP3 inflammasome after a traumatic injury.
Here, the role of the NLRP3 inflammasome in impaired monocyte functionality after a traumatic injury was analyzed.
Measurements and Main Results.
Ex vivo-in vitro stimulation of isolated CD14+ monocytes with lipopolysaccharide (LPS) showed a significantly higher IL-1β secretion in healthy volunteers (HV) compared to trauma patients (TP) after admission.
Reduced IL-1β secretion was paralleled by significantly lowered gene expression of NLRP3 in monocytes from TP compared to those of HV.
Transfection of monocytes with NLRP3-encoding plasmid recovered the functionality of monocytes from TP regarding the IL-1β secretion.
Conclusions.
This study demonstrates that CD14+ monocytes from TP are significantly diminished in their function and that the presence of NLRP3 components is necessary in recovering the ability of monocytes to produce active IL-1β.
This recovery of the NLRP3 inflammasome in monocytes may imply a new target for treatment and therapy of immune suppression after severe injury.
American Psychological Association (APA)
Kany, Shinwan& Horstmann, Johann-Philipp& Sturm, Ramona& Mörs, Katharina& Relja, Borna. 2018. Reduced NLRP3 Gene Expression Limits the IL-1β Cleavage via Inflammasome in Monocytes from Severely Injured Trauma Patients. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1203350
Modern Language Association (MLA)
Kany, Shinwan…[et al.]. Reduced NLRP3 Gene Expression Limits the IL-1β Cleavage via Inflammasome in Monocytes from Severely Injured Trauma Patients. Mediators of Inflammation No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1203350
American Medical Association (AMA)
Kany, Shinwan& Horstmann, Johann-Philipp& Sturm, Ramona& Mörs, Katharina& Relja, Borna. Reduced NLRP3 Gene Expression Limits the IL-1β Cleavage via Inflammasome in Monocytes from Severely Injured Trauma Patients. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1203350
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203350