The Effect and Mechanism of KLF7 in the TLR4NF-κBIL-6 Inflammatory Signal Pathway of Adipocytes
Joint Authors
Zhang, Jun
Xie, Jianxin
Wang, Cuizhe
Ha, Xiaodan
Zhang, Meixiu
Wu, Jinxiu
Deng, Yuchun
Zhang, Xueting
Wang, Jingzhou
Chen, Keru
Feng, Jiale
Zhu, Jiaojiao
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-26
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objective.
To investigate the role and possible molecular mechanism of Krüppel-like factor 7 (KLF7) in the TLR4/NF-κB/IL-6 inflammatory signaling pathway activated by free fatty acids (FFA).
Methods.
The mRNA and protein expression levels of KLF7 and the factors of TLR4/NF-κB/IL-6 inflammatory signal pathways were detected by qRT-PCR and Western blotting after cell culture with different concentrations of palmitic acid (PA).
The expression of KLF7 or TLR4 in adipocytes was upregulated or downregulated; after that, the mRNA and protein expression levels of these key factors were detected.
KLF7 expression was downregulated while PA stimulated adipocytes, and then the mRNA and protein expressions of KLF7/p65 and downstream inflammatory cytokine IL-6 were detected.
The luciferase reporter assay was used to determine whether KLF7 had a transcriptional activation effect on IL-6.
Results.
(1) High concentration of PA can promote the expression of TLR4, KLF7, and IL-6 in adipocytes.
(2) TLR4 positively regulates KLF7 expression in adipocytes.
(3) KLF7 positively regulates IL-6 expression in adipocytes.
(4) PA promotes IL-6 expression via KLF7 in adipocytes.
(5) KLF7 has a transcriptional activation on IL-6.
Conclusion.
PA promotes the expression of the inflammatory cytokine IL-6 by activating the TLR4/KLF7/NF-κB inflammatory signaling pathway.
In addition, KLF7 may directly bind to the IL-6 promoter region and thus activate IL-6.
American Psychological Association (APA)
Zhang, Meixiu& Wang, Cuizhe& Wu, Jinxiu& Ha, Xiaodan& Deng, Yuchun& Zhang, Xueting…[et al.]. 2018. The Effect and Mechanism of KLF7 in the TLR4NF-κBIL-6 Inflammatory Signal Pathway of Adipocytes. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1203354
Modern Language Association (MLA)
Zhang, Meixiu…[et al.]. The Effect and Mechanism of KLF7 in the TLR4NF-κBIL-6 Inflammatory Signal Pathway of Adipocytes. Mediators of Inflammation No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1203354
American Medical Association (AMA)
Zhang, Meixiu& Wang, Cuizhe& Wu, Jinxiu& Ha, Xiaodan& Deng, Yuchun& Zhang, Xueting…[et al.]. The Effect and Mechanism of KLF7 in the TLR4NF-κBIL-6 Inflammatory Signal Pathway of Adipocytes. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1203354
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203354