AKT-GSK3β Signaling Pathway Regulates Mitochondrial Dysfunction-Associated OPA1 Cleavage Contributing to Osteoblast Apoptosis: Preventative Effects of Hydroxytyrosol
Joint Authors
Zhang, Dafeng
Mao, Yixin
Li, Xumin
Ma, Jianfeng
Huang, Shengbin
Sun, Xiaoyu
Cai, W. J.
Chen, Y.
Shi, L. X.
Cheng, H. R.
Banda, I.
Ji, Y. H.
Wang, Y. T.
Dai, P. P.
Ning, X. H.
Zhao, S. F.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-02
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
Oxidative stress (OS) induces osteoblast apoptosis, which plays a crucial role in the initiation and progression of osteoporosis.
Although OS is closely associated with mitochondrial dysfunction, detailed mitochondrial mechanisms underlying OS-induced osteoblast apoptosis have not been thoroughly elucidated to date.
In the present study, we found that mitochondrial abnormalities largely contributed to OS-induced osteoblast apoptosis, as evidenced by enhanced production of mitochondrial reactive oxygen species; considerable reduction in mitochondrial respiratory chain complex activity, mitochondrial membrane potential, and adenosine triphosphate production; abnormality in mitochondrial morphology; and alteration of mitochondrial dynamics.
These mitochondrial abnormalities were primarily mediated by an imbalance in mitochondrial fusion and fission through a protein kinase B- (AKT-) glycogen synthase kinase 3β- (GSK3β-) optic atrophy 1- (OPA1-) dependent mechanism.
Hydroxytyrosol (3,4-dihydroxyphenylethanol (HT)), an important compound in virgin olive oil, significantly prevented OS-induced osteoblast apoptosis.
Specifically, HT inhibited OS-induced mitochondrial dysfunction by decreasing OPA1 cleavage and by increasing AKT and GSK3β phosphorylation.
Together, our results indicate that the AKT-GSK3β signaling pathway regulates mitochondrial dysfunction-associated OPA1 cleavage, which may contribute to OS-induced osteoblast apoptosis.
Moreover, our results suggest that HT could be an effective nutrient for preventing osteoporosis development.
American Psychological Association (APA)
Cai, W. J.& Chen, Y.& Shi, L. X.& Cheng, H. R.& Banda, I.& Ji, Y. H.…[et al.]. 2019. AKT-GSK3β Signaling Pathway Regulates Mitochondrial Dysfunction-Associated OPA1 Cleavage Contributing to Osteoblast Apoptosis: Preventative Effects of Hydroxytyrosol. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-20.
https://search.emarefa.net/detail/BIM-1203504
Modern Language Association (MLA)
Cai, W. J.…[et al.]. AKT-GSK3β Signaling Pathway Regulates Mitochondrial Dysfunction-Associated OPA1 Cleavage Contributing to Osteoblast Apoptosis: Preventative Effects of Hydroxytyrosol. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-20.
https://search.emarefa.net/detail/BIM-1203504
American Medical Association (AMA)
Cai, W. J.& Chen, Y.& Shi, L. X.& Cheng, H. R.& Banda, I.& Ji, Y. H.…[et al.]. AKT-GSK3β Signaling Pathway Regulates Mitochondrial Dysfunction-Associated OPA1 Cleavage Contributing to Osteoblast Apoptosis: Preventative Effects of Hydroxytyrosol. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-20.
https://search.emarefa.net/detail/BIM-1203504
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203504