Structure Characterization and Action Mechanism of an Antiaging New Compound from Gastrodia elata Blume
Joint Authors
Lin, Yanfei
Xiang, Lan
Qi, Jianhua
Pan, Yanjun
Farooq, Umer
Wang, Ying
Osada, Hiroyuki
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A new compound, bis(4-hydroxybenzyl)ether mono-β-L-galactopyranoside (1), was isolated from the rhizome of Gastrodia elata Blume.
Its structure was elucidated using extensive spectroscopic analysis, including 1D and 2D NMR, HR-ESI-TOF-MS, and chemical derivatization.
Compound 1 extended the replicative lifespan of K6001 and the chronological lifespan of YOM36 yeast strains.
To understand the mechanism of action, oxidative stress assessment, reactive oxygen species (ROS) and malondialdehyde (MDA) levels, catalase (CAT) and total glutathione peroxidase (GPx) activity assays, and replicative lifespan assay of sod1, sod2, uth1, and skn7 yeast mutant strains were performed.
Results indicated the significant increase in the survival rate of yeast under oxidative stress after treatment with 1.
ROS and MDA levels were reduced significantly.
Meanwhile, the activity of CAT and GPx was significantly increased.
The lifespan of sod1, sod2, uth1, and skn7 mutants of K6001 was not affected by 1.
Furthermore, we investigated the gene expression related to longevity after administrating 1.
The significant increase of Sir2 and reduction of Uth1 gene expression in the 1-treated group were observed.
These results indicated that antioxidative stress played an important role in the antiaging effect of 1; Sir2 and Uth1 genes were involved in antiaging effects of 1.
American Psychological Association (APA)
Farooq, Umer& Pan, Yanjun& Lin, Yanfei& Wang, Ying& Osada, Hiroyuki& Xiang, Lan…[et al.]. 2019. Structure Characterization and Action Mechanism of an Antiaging New Compound from Gastrodia elata Blume. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1204167
Modern Language Association (MLA)
Farooq, Umer…[et al.]. Structure Characterization and Action Mechanism of an Antiaging New Compound from Gastrodia elata Blume. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1204167
American Medical Association (AMA)
Farooq, Umer& Pan, Yanjun& Lin, Yanfei& Wang, Ying& Osada, Hiroyuki& Xiang, Lan…[et al.]. Structure Characterization and Action Mechanism of an Antiaging New Compound from Gastrodia elata Blume. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1204167
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204167