Sedoheptulose-1,7-bisphospate Accumulation and Metabolic Anomalies in Hepatoma Cells Exposed to Oxidative Stress
Joint Authors
Cheng, Mei-Ling
Ho, Hung-Yao
Lin, Jui-Fen
Huang, Cheng-Yu
Li, Guan-Jie
Shih, Lu-Min
Chiu, Daniel Tsun-Yee
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-13
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
We have previously shown that GSH depletion alters global metabolism of cells.
In the present study, we applied a metabolomic approach for studying the early changes in metabolism in hydrogen peroxide- (H2O2-) treated hepatoma cells which were destined to die.
Levels of fructose 1,6-bisphosphate and an unusual metabolite, sedoheptulose 1,7-bisphosphate (S-1,7-BP), were elevated in hepatoma Hep G2 cells.
Deficiency in G6PD activity significantly reduced S-1,7-BP formation, suggesting that S-1,7-BP is formed in the pentose phosphate pathway as a response to oxidative stress.
Additionally, H2O2 treatment significantly increased the level of nicotinamide adenine dinucleotide phosphate (NADP+) and reduced the levels of ATP and NAD+.
Severe depletion of ATP and NAD+ in H2O2-treated Hep G2 cells was associated with cell death.
Inhibition of PARP-mediated NAD+ depletion partially protected cells from death.
Comparison of metabolite profiles of G6PD-deficient cells and their normal counterparts revealed that changes in GSH and GSSG per se do not cause cell death.
These findings suggest that the failure of hepatoma cells to maintain energy metabolism in the midst of oxidative stress may cause cell death.
American Psychological Association (APA)
Cheng, Mei-Ling& Lin, Jui-Fen& Huang, Cheng-Yu& Li, Guan-Jie& Shih, Lu-Min& Chiu, Daniel Tsun-Yee…[et al.]. 2019. Sedoheptulose-1,7-bisphospate Accumulation and Metabolic Anomalies in Hepatoma Cells Exposed to Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1204352
Modern Language Association (MLA)
Cheng, Mei-Ling…[et al.]. Sedoheptulose-1,7-bisphospate Accumulation and Metabolic Anomalies in Hepatoma Cells Exposed to Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1204352
American Medical Association (AMA)
Cheng, Mei-Ling& Lin, Jui-Fen& Huang, Cheng-Yu& Li, Guan-Jie& Shih, Lu-Min& Chiu, Daniel Tsun-Yee…[et al.]. Sedoheptulose-1,7-bisphospate Accumulation and Metabolic Anomalies in Hepatoma Cells Exposed to Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1204352
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204352