Increased HIF-1α in Knee Osteoarthritis Aggravate Synovial Fibrosis via Fibroblast-Like Synoviocyte Pyroptosis
Joint Authors
Xing, Runlin
Wang, Peimin
Zhang, Li
Huang, Zhengquan
Zhang, Nongshan
Zhang, Li
Li, Xiaochen
Yin, Songjiang
Mao, Jun
Mei, Wei
Ding, Liang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Fibroblast-like synoviocytes (FLSs) are the main effector cells of knee osteoarthritis (KOA) synovial fibrosis.
Our last report showed that NLRP1 and NLRP3 inflammasomes may mediate LPS/ATP-induced FLSs pyroptosis in KOA.
In the present study, we found an elevated hypoxia-inducible factor-1α (HIF-1α) level in the synovial tissue of KOA model rats, and inhibiting the increase of HIF-1α could improve synovial fibrosis in rats.
Subsequently, we established LPS/ATP-induced model in FLSs mimicking the inflammatory environment of KOA.
FLSs transfected with siRNA HIF-1α showed a reduced cell death; meanwhile, the relative expression of pyroptosis-related proteins was also downregulated.
Additionally, FLSs transfected with or without siRNA GSDMD were exposed to hypoxia.
GSDMD silencing can significantly reduce both gene and protein levels of fibrogenic markers transforming growth factor-β (TGF-β), procollagen-lysine, 2-oxoglutarate 5-dioxygenase2 (PLOD2), collagen type I α1 chain (COL1A1), and tissue inhibitor of metalloproteinases 1 (TIMP1).
Taken together, our findings indicate that increased HIF-1α is highly involved in the KOA synovial fibrosis.
Moreover, elevated HIF-1α may aggravate synovial fibrosis via FLS pyroptosis.
American Psychological Association (APA)
Zhang, Li& Zhang, Li& Huang, Zhengquan& Xing, Runlin& Li, Xiaochen& Yin, Songjiang…[et al.]. 2019. Increased HIF-1α in Knee Osteoarthritis Aggravate Synovial Fibrosis via Fibroblast-Like Synoviocyte Pyroptosis. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204547
Modern Language Association (MLA)
Zhang, Li…[et al.]. Increased HIF-1α in Knee Osteoarthritis Aggravate Synovial Fibrosis via Fibroblast-Like Synoviocyte Pyroptosis. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1204547
American Medical Association (AMA)
Zhang, Li& Zhang, Li& Huang, Zhengquan& Xing, Runlin& Li, Xiaochen& Yin, Songjiang…[et al.]. Increased HIF-1α in Knee Osteoarthritis Aggravate Synovial Fibrosis via Fibroblast-Like Synoviocyte Pyroptosis. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204547
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204547