Inhibitory Effect of Delphinidin on Oxidative Stress Induced by H2O2 in HepG2 Cells
Joint Authors
Xu, Jingjing
Ren, Guofeng
Yang, Rengui
Chen, Jingfang
Xiang, Xiaojing
Qin, Hong
Chen, Jihua
Zhang, Yanwei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-21
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Chronic liver diseases (CLDs) are correlated with oxidative stress induced by the accumulation of intracellular reactive oxygen species (ROS).
In this study, we employed HepG2, a human liver carcinoma cell line containing many antioxidant enzymes, to explore the function of delphinidin against oxidative stress induced by H2O2 and to provide scientific data of the molecular mechanism.
Cells were pretreated with different concentrations of delphinidin (10 μmol/L, 20 μmol/L, and 40 μmol/L) for 2 h before treatment with 750 μM H2O2 for 1 h.
The results showed that H2O2 decreased the survival rate of HepG2 cells and increased the level of ROS, but delphinidin pretreatment could possess the opposite result.
At the same time, the expression of Nrf2 was enhanced by the delphinidin pretreatment.
This was because delphinidin promoted Nrf2 nuclear translocation and inhibited its degradation, which led to the increase expression of antioxidant protein HO-1 (Nrf2-related phase II enzyme heme oxygenase-1).
Besides, we found that delphinidin could significantly alleviate the reduction of Nrf2 protein levels and the accumulation of intracellular ROS levels in Nrf2 knockdown HepG2 cells.
In conclusion, our study suggested that delphinidin, as an effective antioxidant, protected HepG2 cells from oxidative stress by regulating the expression of Nrf2/HO-1.
American Psychological Association (APA)
Xu, Jingjing& Zhang, Yanwei& Ren, Guofeng& Yang, Rengui& Chen, Jingfang& Xiang, Xiaojing…[et al.]. 2020. Inhibitory Effect of Delphinidin on Oxidative Stress Induced by H2O2 in HepG2 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1204599
Modern Language Association (MLA)
Xu, Jingjing…[et al.]. Inhibitory Effect of Delphinidin on Oxidative Stress Induced by H2O2 in HepG2 Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1204599
American Medical Association (AMA)
Xu, Jingjing& Zhang, Yanwei& Ren, Guofeng& Yang, Rengui& Chen, Jingfang& Xiang, Xiaojing…[et al.]. Inhibitory Effect of Delphinidin on Oxidative Stress Induced by H2O2 in HepG2 Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1204599
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204599