HIF1α-Induced Glycolysis in Macrophage Is Essential for the Protective Effect of Ouabain during Endotoxemia
Joint Authors
Bo, Lulong
Bian, Jinjun
Shao, Chao
Lin, Shengwei
Liu, Sudan
Jin, Peipei
Lu, Wenbin
Li, Na
Zhang, Yan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Ouabain, a steroid binding to the Na+/K+-ATPase, has several pharmacological effects.
In addition to the recognized effects of blood pressure, there is more convincing evidence suggesting that ouabain is involved in immunologic functions and inflammation.
Hypoxia-inducible factor 1α (HIF-1α) is a metabolic regulator which plays a considerable role in immune responses.
Previous studies had shown that HIF-1α-induced glycolysis results in functional reshaping in macrophages.
In this study, we investigated the role of glycolytic pathway activation in the anti-inflammatory effect of ouabain.
We found that ouabain is involved in anti-inflammatory effects both in vivo and in vitro.
Additionally, ouabain can inhibit LPS-induced upregulation of GLUT1 and HK2 at the transcriptional level.
GM-CSF pretreatment almost completely reversed the inhibitory effect of ouabain on LPS-induced release of proinflammatory cytokines.
Alterations in glycolytic pathway activation were required for the anti-inflammatory effect of ouabain.
Ouabain can significantly inhibit the upregulation of HIF-1α at the protein level.
Our results also revealed that the overexpression of HIF-1α can reverse the anti-inflammatory effect of ouabain.
Thus, we conclude that the HIF-1α-dependent glycolytic pathway is essential for the anti-inflammatory effect of ouabain.
American Psychological Association (APA)
Shao, Chao& Lin, Shengwei& Liu, Sudan& Jin, Peipei& Lu, Wenbin& Li, Na…[et al.]. 2019. HIF1α-Induced Glycolysis in Macrophage Is Essential for the Protective Effect of Ouabain during Endotoxemia. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1205009
Modern Language Association (MLA)
Shao, Chao…[et al.]. HIF1α-Induced Glycolysis in Macrophage Is Essential for the Protective Effect of Ouabain during Endotoxemia. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1205009
American Medical Association (AMA)
Shao, Chao& Lin, Shengwei& Liu, Sudan& Jin, Peipei& Lu, Wenbin& Li, Na…[et al.]. HIF1α-Induced Glycolysis in Macrophage Is Essential for the Protective Effect of Ouabain during Endotoxemia. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1205009
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205009