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(–)-Epicatechin Modulates Mitochondrial Redox in Vascular Cell Models of Oxidative Stress
Joint Authors
Keller, Amy C.
Hull, Sara E.
Elajaili, Hanan
Johnston, Aspen
Knaub, Leslie A.
Chun, Ji Hye
Walker, Lori
Nozik-Grayck, Eva
Reusch, Jane E. B.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-09
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Diabetes mellitus affects 451 million people worldwide, and people with diabetes are 3-5 times more likely to develop cardiovascular disease.
In vascular tissue, mitochondrial function is important for vasoreactivity.
Diabetes-mediated generation of excess reactive oxygen species (ROS) may contribute to vascular dysfunction via damage to mitochondria and regulation of endothelial nitric oxide synthase (eNOS).
We have identified (–)-epicatechin (EPICAT), a plant compound and known vasodilator, as a potential therapy.
We hypothesized that mitochondrial ROS in cells treated with antimycin A (AA, a compound targeting mitochondrial complex III) or high glucose (HG, global perturbation) could be normalized by EPICAT, and correlate with improved mitochondrial dynamics and cellular signaling.
Human umbilical vein endothelial cells (HUVEC) were treated with HG, AA, and/or 0.1 or 1.0 μM of EPICAT.
Mitochondrial and cellular superoxide, mitochondrial respiration, and cellular signaling upstream of mitochondrial function were assessed.
EPICAT at 1.0 μM significantly attenuated mitochondrial superoxide in HG-treated cells.
At 0.1 μM, EPICAT nonsignificantly increased mitochondrial respiration, agreeing with previous reports.
EPICAT significantly increased complex I expression in AA-treated cells, and 1.0 μM EPICAT significantly decreased mitochondrial complex V expression in HG-treated cells.
No significant effects were seen on either AMPK or eNOS expression.
Our study suggests that EPICAT is useful in mitigating moderate ROS concentrations from a global perturbation and may modulate mitochondrial complex activity.
Our data illustrate that EPICAT acts in the cell in a dose-dependent manner, demonstrating hormesis.
American Psychological Association (APA)
Keller, Amy C.& Hull, Sara E.& Elajaili, Hanan& Johnston, Aspen& Knaub, Leslie A.& Chun, Ji Hye…[et al.]. 2020. (–)-Epicatechin Modulates Mitochondrial Redox in Vascular Cell Models of Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1205112
Modern Language Association (MLA)
Keller, Amy C.…[et al.]. (–)-Epicatechin Modulates Mitochondrial Redox in Vascular Cell Models of Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1205112
American Medical Association (AMA)
Keller, Amy C.& Hull, Sara E.& Elajaili, Hanan& Johnston, Aspen& Knaub, Leslie A.& Chun, Ji Hye…[et al.]. (–)-Epicatechin Modulates Mitochondrial Redox in Vascular Cell Models of Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1205112
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205112