Sodium Butyrate Inhibits Neovascularization Partially via TNXIPVEGFR2 Pathway
Joint Authors
Chen, Haoyu
Xiao, Xiaoqiang
Chen, Min
Xu, Yanxuan
Huang, Shaofen
Liang, Jiajian
Cao, Yingjie
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-21
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Control of neovascularization with small molecules is a promising tactics.
Here, we tested the roles of sodium butyrate (NaBu) on the neovascularization and primary explained its underlining molecular links.
We used models including cell and ex vivo culture of choroid and mouse, as well as the biochemical and cellular techniques, to confirm our hypothesis.
We found that treating HUVEC cells with NaBu (both 2.5 mM and 5 mM) significantly inhibited its ability in tube formation and proliferation.
This inhibitory effect was also observed in choroid sprouting experiments, compared to the control.
Interestingly, the choroid sprouting suppressed by NaBu can proliferate again after removing it, indicating that the cell cycle progression might be arrested.
The laser-induced choroid neovascularization (CNV) was significantly alleviated by assessing the CNV size (decreased to 0.73 fold) in contrast with the vehicle control group after 2.5 mM NaBu injection for 7 days.
Mechanistically, we found an enhanced TXNIP expression in response to NaBu treatment in all the three models.
Overexpressing TXNIP in HUVEC cells blocked its tube formation and inhibited its proliferation; on the other hand, knocking down its expression with shRNA reversed those phenotypes in context of NaBu treatment.
Further investigation showed the expression of VEGF receptor 2 (VEGFR2) in HUVEC cells was regulated by TXNIP undergoing NaBu treatment.
We therefore argued that NaBu inhibited neovascularization partially through TXNIP-regulated VEGFR2 signal pathway.
American Psychological Association (APA)
Xiao, Xiaoqiang& Chen, Min& Xu, Yanxuan& Huang, Shaofen& Liang, Jiajian& Cao, Yingjie…[et al.]. 2020. Sodium Butyrate Inhibits Neovascularization Partially via TNXIPVEGFR2 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1205120
Modern Language Association (MLA)
Xiao, Xiaoqiang…[et al.]. Sodium Butyrate Inhibits Neovascularization Partially via TNXIPVEGFR2 Pathway. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1205120
American Medical Association (AMA)
Xiao, Xiaoqiang& Chen, Min& Xu, Yanxuan& Huang, Shaofen& Liang, Jiajian& Cao, Yingjie…[et al.]. Sodium Butyrate Inhibits Neovascularization Partially via TNXIPVEGFR2 Pathway. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1205120
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205120