Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation

Joint Authors

Ma, Zengchun
Tan, Hongling
Wang, Ningning
Wang, Jia
Zhou, Wei
You, Wenting
Ge, Yunxuan
Wang, Yuguang
Gao, Yue

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-22, 22 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-31

Country of Publication

Egypt

No. of Pages

22

Main Subjects

Biology

Abstract EN

Ophiopogonin D (OPD), a compound from the Chinese herb Radix Ophiopogonis, reportedly induces increased levels of cytochrome P450 2J3 (CYP2J3)/epoxyeicosatrienoic acids (EETs) and Ca2+ in rat cardiomyocytes.

Little is known regarding the specific mechanism between CYP2J3 and Ca2+ homeostasis.

Here, we investigated whether CYP2J3 is involved in the protective action of OPD on the myocardium by activating the Ca2+ homeostasis-related protein complex (SERCA2a and PLB) in H9c2 rat cardiomyoblast cells.

The interaction between SERCA2a and PLB was measured using fluorescence resonance energy transfer.

OPD attenuated heart failure and catalyzed the active transport of Ca2+ into the sarcoplasmic reticulum by inducing the phosphorylation of PLB and promoting the SERCA2a activity.

These beneficial effects of OPD on heart failure were abolished after knockdown of CYP2J3 in a model of heart failure.

Together, our results identify CYP2J3 as a critical intracellular target for OPD and unravel a mechanism of CYP2J3-dependent regulation of intracellular Ca2+.

American Psychological Association (APA)

Wang, Jia& You, Wenting& Wang, Ningning& Zhou, Wei& Ma, Zengchun& Tan, Hongling…[et al.]. 2020. Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-22.
https://search.emarefa.net/detail/BIM-1205786

Modern Language Association (MLA)

Ma, Zengchun…[et al.]. Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-22.
https://search.emarefa.net/detail/BIM-1205786

American Medical Association (AMA)

Wang, Jia& You, Wenting& Wang, Ningning& Zhou, Wei& Ma, Zengchun& Tan, Hongling…[et al.]. Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-22.
https://search.emarefa.net/detail/BIM-1205786

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1205786