6,8-Diprenylorobol Induces Apoptosis in Human Hepatocellular Carcinoma Cells via Activation of FOXO3 and Inhibition of CYP2J2
Joint Authors
Lee, Chang Min
Park, See-Hyoung
Jang, Su-Nyeong
Shon, Jong Cheol
Wu, Zhexue
Park, Kyungmoon
Liu, Kwang-Hyeon
Lee, Jongsung
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-19
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
6,8-Diprenylorobol is a phytochemical derived from the roots of Glycyrrhiza uralensis Fisch.
6,8-Diprenylorobol exhibits several biological activities, but the effects of 6,8-diprenylorobol on cancers have been hardly investigated.
This study is aimed at elucidating the anticancer effect and working mechanism of 6,8-diprenylorobol in HepG2 and Huh-7, two kinds of human hepatocellular carcinoma (HCC) cell lines.
WST-1, cell counting, and colony formation assays and morphological change analysis showed that 6,8-diprenylorobol treatment decreased the cell viability and proliferation rate.
Cell cycle analysis indicated that 6,8-diprenylorobol treatment increased the population of the G1/0 stage.
Annexin V/PI double staining and TUNEL analysis showed that 6,8-diprenylorobol treatment increased the apoptotic cell population and DNA fragmentation.
Western blot analysis showed that 6,8-diprenylorobol treatment increased the expression of cleaved PARP1, cleaved caspase-3, FOXO3, Bax, Bim, p21, and p27 but decreased the expression of Bcl2 and BclXL.
Interestingly, 6,8-diprenylorobol inhibited CYP2J2-mediated astemizole O-demethylation and ebastine hydroxylase activities with Ki values of 9.46 and 2.61 μM, respectively.
CYP2J2 siRNA transfection enhanced the anticancer effect of 6,8-diprenylorobol in HepG2 and Huh-7 cells through the downregulation of CYP2J2 protein expression and upregulation of FOXO3.
Taken together, this study proposes that 6,8-diprenylorobol treatment may be a useful therapeutic option against HCC by targeting CYP2J2 and FOXO3.
American Psychological Association (APA)
Lee, Chang Min& Lee, Jongsung& Jang, Su-Nyeong& Shon, Jong Cheol& Wu, Zhexue& Park, Kyungmoon…[et al.]. 2020. 6,8-Diprenylorobol Induces Apoptosis in Human Hepatocellular Carcinoma Cells via Activation of FOXO3 and Inhibition of CYP2J2. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-19.
https://search.emarefa.net/detail/BIM-1205871
Modern Language Association (MLA)
Lee, Chang Min…[et al.]. 6,8-Diprenylorobol Induces Apoptosis in Human Hepatocellular Carcinoma Cells via Activation of FOXO3 and Inhibition of CYP2J2. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-19.
https://search.emarefa.net/detail/BIM-1205871
American Medical Association (AMA)
Lee, Chang Min& Lee, Jongsung& Jang, Su-Nyeong& Shon, Jong Cheol& Wu, Zhexue& Park, Kyungmoon…[et al.]. 6,8-Diprenylorobol Induces Apoptosis in Human Hepatocellular Carcinoma Cells via Activation of FOXO3 and Inhibition of CYP2J2. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-19.
https://search.emarefa.net/detail/BIM-1205871
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205871