Chicoric Acid Ameliorates Nonalcoholic Fatty Liver Disease via the AMPKNrf2NFκB Signaling Pathway and Restores Gut Microbiota in High-Fat-Diet-Fed Mice
Joint Authors
Ding, Xiaoqin
Jian, Tunyu
Li, Jiawei
Lv, Han
Tong, Bei
Li, Jing
Meng, Xiuhua
Ren, Bingru
Chen, Jian
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-04
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
This study examines the effects of chicoric acid (CA) on nonalcoholic fatty liver disease (NAFLD) in high-fat-diet- (HFD-) fed C57BL/6 mice.
CA treatment decreased body weight and white adipose weight, mitigated hyperglycemia and dyslipidemia, and reduced hepatic steatosis in HFD-fed mice.
Moreover, CA treatment reversed HFD-induced oxidative stress and inflammation both systemically and locally in the liver, evidenced by the decreased serum malondialdehyde (MDA) abundance, increased serum superoxide dismutase (SOD) activity, lowered in situ reactive oxygen species (ROS) in the liver, decreased serum and hepatic inflammatory cytokine levels, and reduced hepatic inflammatory cell infiltration in HFD-fed mice.
In addition, CA significantly reduced lipid accumulation and oxidative stress in palmitic acid- (PA-) treated HepG2 cells.
In particular, we identified AMPK as an activator of Nrf2 and an inactivator of NFκB.
CA upregulated AMPK phosphorylation, the nuclear protein level of Nrf2, and downregulated NFκB protein level both in HFD mice and PA-treated HepG2 cells.
Notably, AMPK inhibitor compound C blocked the regulation of Nrf2 and NFκB, as well as ROS overproduction mediated by CA in PA-treated HepG2 cells, while AMPK activator AICAR mimicked the effects of CA.
Similarly, Nrf2 inhibitor ML385 partly blocked the regulation of antioxidative genes and ROS overproduction by CA in PA-treated HepG2 cells.
Interestingly, high-throughput pyrosequencing of 16S rRNA suggested that CA could increase Firmicutes-to-Bacteroidetes ratio and modify gut microbial composition towards a healthier microbial profile.
In summary, CA plays a preventative role in the amelioration of oxidative stress and inflammation via the AMPK/Nrf2/NFκB signaling pathway and shapes gut microbiota in HFD-induced NAFLD.
American Psychological Association (APA)
Ding, Xiaoqin& Jian, Tunyu& Li, Jiawei& Lv, Han& Tong, Bei& Li, Jing…[et al.]. 2020. Chicoric Acid Ameliorates Nonalcoholic Fatty Liver Disease via the AMPKNrf2NFκB Signaling Pathway and Restores Gut Microbiota in High-Fat-Diet-Fed Mice. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1206122
Modern Language Association (MLA)
Ding, Xiaoqin…[et al.]. Chicoric Acid Ameliorates Nonalcoholic Fatty Liver Disease via the AMPKNrf2NFκB Signaling Pathway and Restores Gut Microbiota in High-Fat-Diet-Fed Mice. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-20.
https://search.emarefa.net/detail/BIM-1206122
American Medical Association (AMA)
Ding, Xiaoqin& Jian, Tunyu& Li, Jiawei& Lv, Han& Tong, Bei& Li, Jing…[et al.]. Chicoric Acid Ameliorates Nonalcoholic Fatty Liver Disease via the AMPKNrf2NFκB Signaling Pathway and Restores Gut Microbiota in High-Fat-Diet-Fed Mice. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1206122
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206122