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Effect of PKCNF-κB on the Regulation of P2X3 Receptor in Dorsal Root Ganglion in Rats with Sciatic Nerve Injury
Joint Authors
Yuan, Jie
Zhang, Qin
Li, Xue
Yu, Xuan
Qin, Bangyong
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Protein kinase C (PKC), nuclear factor-kappa B p65 (NF-κB p65), and P2X3 receptor (P2X3R) play significant roles in the sensitization and transduction of nociceptive signals, which are considered as potential targets for the treatment of neuropathic pain.
However, the mechanisms and relationships among them have not been clearly clarified.
Methods.
80 rats were randomized and divided into 10 groups (n = 8).
Sciatic chronic constriction injury (CCI) rats were intrathecally administered with bisindolylmaleimide I (GF109203X), a PKC-selective antagonist once a day, or pyrrolidine dithiocarbamate (PDTC), an NF-κB inhibitor twice a day.
Sham-operated rats were intrathecally administered with saline.
Thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) were evaluated in all the groups before CCI operation (baseline) and on the 1st, 3rd, 7th, 10th, and 14th day after CCI operation.
Protein levels of p-PKCα, p-NF-κB p65, and P2X3R were analyzed in the CCI ipsilateral L4–6 dorsal root ganglions (DRGs).
Results.
Intrathecal injection of GF109203X or PDTC alleviated the TWL and MWT in the following 2 weeks after CCI surgery.
The protein levels of p-PKCα, p-NF-κB p65, and P2X3R in the ipsilateral DRGs significantly increased after CCI operation, which could be partly reversed by intrathecal administration of GF109203X or PDTC.
Conclusion.
The upregulation of p-PKCα, p-NF-κB p65, and P2X3R expression in the DRGs of CCI rats was involved in the occurrence and development of neuropathic pain.
Phosphorylated PKCα and phosphorylated NF-κB p65 regulated with each other.
Phosphorylated NF-κB p65 and PKCα have a mutual regulation relationship with P2X3R, respectively, while the specific regulatory mechanism needs further research.
American Psychological Association (APA)
Li, Xue& Yuan, Jie& Yu, Xuan& Zhang, Qin& Qin, Bangyong. 2020. Effect of PKCNF-κB on the Regulation of P2X3 Receptor in Dorsal Root Ganglion in Rats with Sciatic Nerve Injury. Pain Research and Management،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1206957
Modern Language Association (MLA)
Li, Xue…[et al.]. Effect of PKCNF-κB on the Regulation of P2X3 Receptor in Dorsal Root Ganglion in Rats with Sciatic Nerve Injury. Pain Research and Management No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1206957
American Medical Association (AMA)
Li, Xue& Yuan, Jie& Yu, Xuan& Zhang, Qin& Qin, Bangyong. Effect of PKCNF-κB on the Regulation of P2X3 Receptor in Dorsal Root Ganglion in Rats with Sciatic Nerve Injury. Pain Research and Management. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1206957
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206957