Changes of KEAP1NRF2 and IKBNF-κB Expression Levels Induced by Cell-Free DNA in Different Cell Types
Joint Authors
Izevskaya, Vera L.
Porokhovnik, Lev
Ershova, Elizaveta
Veiko, Natalia
Kutsev, S. I.
Martynov, A. V.
Kaliyanov, Andrew A.
Sergeeva, Vasilina A.
Abramova, Margarita S.
Kameneva, L. V.
Veiko, V. P.
Kostyuk, Svetlana V.
Malinovskaya, Elena M.
Dolgikh, Olga A.
Pisarev, Vladimir M.
Filev, Anton D.
Chudakova, Julia M.
Konkova, Marina S.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-20
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Cell-free DNA (cfDNA) is a circulating DNA of nuclear and mitochondrial origin mainly derived from dying cells.
Recent studies have shown that cfDNA is a stress signaling DAMP (damage-associated molecular pattern) molecule.
We report here that the expression profiles of cfDNA-induced factors NRF2 and NF-κB are distinct depending on the target cell’s type and the GC-content and oxidation rate of the cfDNA.
Stem cells (MSC) have shown higher expression of NRF2 without inflammation in response to cfDNA.
In contrast, inflammatory response launched by NF-κB was dominant in differentiated cells HUVEC, MCF7, and fibroblasts, with a possibility of transition to massive apoptosis.
In each cell type examined, the response for oxidized cfDNA was more acute with higher peak intensity and faster resolution than that for nonoxidized cfDNA.
GC-rich nonoxidized cfDNA evoked a weaker and prolonged response with proinflammatory component (NF-κB) as predominant.
The exploration of apoptosis rates after adding cfDNA showed that cfDNA with moderately increased GC-content and lightly oxidized DNA promoted cell survival in a hormetic manner.
Novel potential therapeutic approaches are proposed, which depend on the current cfDNA content: either preconditioning with low doses of cfDNA before a planned adverse impact or eliminating (binding, etc.) cfDNA when its content has already become high.
American Psychological Association (APA)
Kostyuk, Svetlana V.& Porokhovnik, Lev& Ershova, Elizaveta& Malinovskaya, Elena M.& Konkova, Marina S.& Kameneva, L. V.…[et al.]. 2018. Changes of KEAP1NRF2 and IKBNF-κB Expression Levels Induced by Cell-Free DNA in Different Cell Types. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1210808
Modern Language Association (MLA)
Kostyuk, Svetlana V.…[et al.]. Changes of KEAP1NRF2 and IKBNF-κB Expression Levels Induced by Cell-Free DNA in Different Cell Types. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-17.
https://search.emarefa.net/detail/BIM-1210808
American Medical Association (AMA)
Kostyuk, Svetlana V.& Porokhovnik, Lev& Ershova, Elizaveta& Malinovskaya, Elena M.& Konkova, Marina S.& Kameneva, L. V.…[et al.]. Changes of KEAP1NRF2 and IKBNF-κB Expression Levels Induced by Cell-Free DNA in Different Cell Types. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1210808
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1210808