Partial Inhibition of HO-1 Attenuates HMP-Induced Hepatic Regeneration against Liver Injury in Rats
Joint Authors
Zhou, Lin
Yin, Shengyong
He, Ning
Jia, Jun-Jun
Li, Jian-Hui
He, Yong
Liang, Ruo-peng
Jiang, Li
Liu, Jing-feng
Xu, Kang-di
Zhang, Zhi-hao
Zheng, Shusen
Xie, Haiyang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We found better liver graft regeneration with hypothermic machine perfusion (HMP) compared with static cold storage (SCS) for the first time in our pilot study, but the underlying mechanisms are unknown.
Upregulated heme oxygenase- (HO-) 1 expression has been reported to play a pivotal role in promoting hepatocyte proliferation.
Here, we evaluated the novel role of HO-1 in liver graft protection by HMP.
Rats with a heterozygous knockout of HO-1 (HO-1+/−) were generated and subjected to 3 h of SCS or HMP pre-half-size liver transplantation (HSLT) in vivo or 6 h of SCS or HMP in vitro; control rats were subjected to the same conditions (HO-1+/+).
We found that HSLT induced significant elevation of the HO-1 protein level in the regenerated liver and that HO-1 haplodeficiency resulted in decreased proliferation post-HSLT.
Compared with SCS, HMP induced significant elevation of the HO-1 protein level along with better liver recovery, both of which were reduced by HO-1 haplodeficiency.
HO-1 haplodeficiency-induced decreased proliferation was responsible for the attenuated regenerative ability of HMP.
Mechanistically, HO-1 haploinsufficiency resulted in suppression of hepatocyte growth factor (HGF)/Akt activity.
Our results suggest that inhibition of HO-1 mitigates HMP-induced liver recovery effects related to proliferation, in part, by downregulating the HGF-Akt axis.
American Psychological Association (APA)
He, Ning& Jia, Jun-Jun& Xie, Haiyang& Li, Jian-Hui& He, Yong& Yin, Shengyong…[et al.]. 2018. Partial Inhibition of HO-1 Attenuates HMP-Induced Hepatic Regeneration against Liver Injury in Rats. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1212353
Modern Language Association (MLA)
He, Ning…[et al.]. Partial Inhibition of HO-1 Attenuates HMP-Induced Hepatic Regeneration against Liver Injury in Rats. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1212353
American Medical Association (AMA)
He, Ning& Jia, Jun-Jun& Xie, Haiyang& Li, Jian-Hui& He, Yong& Yin, Shengyong…[et al.]. Partial Inhibition of HO-1 Attenuates HMP-Induced Hepatic Regeneration against Liver Injury in Rats. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1212353
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212353