Pharmaceutical Induction of PGC-1α Promotes Retinal Pigment Epithelial Cell Metabolism and Protects against Oxidative Damage
Joint Authors
Satish, Sangeeta
Philipose, Hannah
Rosales, Mariana Aparecida Brunini
Saint-Geniez, Magali
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Retinal pigment epithelium (RPE) dysfunction due to accumulation of reactive oxygen species and oxidative damage is a key event in the development of age-related macular degeneration (AMD).
Here, we examine the therapeutic potential of ZLN005, a selective PGC-1α transcriptional regulator, in protecting RPE from cytotoxic oxidative damage.
Gene expression analysis on ARPE-19 cells treated with ZLN005 shows robust upregulation of PGC-1α and its associated transcription factors, antioxidant enzymes, and mitochondrial genes.
Energetic profiling shows that ZLN005 treatment enhances RPE mitochondrial function by increasing basal and maximal respiration rates, and spare respiratory capacity.
In addition, ZLN005 robustly protects ARPE-19 cells from cell death caused by H2O2, ox-LDL, and NaIO3 without exhibiting any cytotoxicity under basal conditions.
ZLN005 protection against H2O2-mediated cell death was lost in PGC-1α-silenced cells.
Our data indicates that ZLN005 efficiently protects RPE cells from oxidative damage through selective induction of PGC-1α and its target antioxidant enzymes.
ZLN005 may serve as a novel therapeutic agent for retinal diseases associated with RPE dystrophies.
American Psychological Association (APA)
Satish, Sangeeta& Philipose, Hannah& Rosales, Mariana Aparecida Brunini& Saint-Geniez, Magali. 2018. Pharmaceutical Induction of PGC-1α Promotes Retinal Pigment Epithelial Cell Metabolism and Protects against Oxidative Damage. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1212366
Modern Language Association (MLA)
Satish, Sangeeta…[et al.]. Pharmaceutical Induction of PGC-1α Promotes Retinal Pigment Epithelial Cell Metabolism and Protects against Oxidative Damage. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1212366
American Medical Association (AMA)
Satish, Sangeeta& Philipose, Hannah& Rosales, Mariana Aparecida Brunini& Saint-Geniez, Magali. Pharmaceutical Induction of PGC-1α Promotes Retinal Pigment Epithelial Cell Metabolism and Protects against Oxidative Damage. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1212366
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212366