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IFNα-Expressing Amniotic Fluid-Derived Mesenchymal Stem Cells Migrate to and Suppress HeLa Cell-Derived Tumors in a Mouse Model
Joint Authors
Li, Liru
Wang, Dejun
Guo, Qiuyan
Liang, Tian
Zhou, Jun
Cheng, Yan
Zhang, Guangmei
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-02
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Background.
Immunotherapy for cervical cancer with type I interferon (IFN) is limited because of the cytotoxicity that accompanies the high doses that are administered.
In this study, we investigated the utilization of amniotic fluid-derived mesenchymal stem cells (AF-MSCs) as a means for delivering IFNα to local tumor sites for the suppression of cervical cancer in a mouse model using HeLa cell xenografts.
Methods.
The tumor tropism ability of AF-MSCs and AF-MSCs genetically modified to overexpress IFNα (IFNα-AF-MSCs) was examined through Transwell in vitro and through fluorescent images and immunohistochemistry in a mouse model.
Tumor size and tumor apoptosis were observed to evaluate the efficacy of the targeting therapy.
Mechanistically, tumor cell apoptosis was detected by cytometry and TUNEL, and oncogenic proteins c-Myc, p53, and Bcl-2 as well as microvessel density were detected by immunohistochemistry.
Results.
In this model, intravenously injected AF-MSCs selectively migrated to the tumor sites, participated in tumor construction, and promoted tumor growth.
After being genetically modified to overexpress IFNα, the IFNα-AF-MSCs maintained their tumor tropism but could significantly suppress tumor growth.
The restrictive efficacy of IFNα-AF-MSCs was associated with the suppression of angiogenesis, inhibition of tumor cell proliferation, and induction of apoptosis in tumor cells.
Neither AF-MSCs nor IFNα-AF-MSCs trigger tumor formation.
Conclusions.
IFNα-AF-MSC-based therapy is feasible and shows potential for treating cervical cancer, suggesting that AF-MSCs may be promising vehicles for delivering targeted anticancer therapy.
American Psychological Association (APA)
Zhou, Jun& Liang, Tian& Wang, Dejun& Li, Liru& Cheng, Yan& Guo, Qiuyan…[et al.]. 2018. IFNα-Expressing Amniotic Fluid-Derived Mesenchymal Stem Cells Migrate to and Suppress HeLa Cell-Derived Tumors in a Mouse Model. Stem Cells International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213120
Modern Language Association (MLA)
Zhou, Jun…[et al.]. IFNα-Expressing Amniotic Fluid-Derived Mesenchymal Stem Cells Migrate to and Suppress HeLa Cell-Derived Tumors in a Mouse Model. Stem Cells International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1213120
American Medical Association (AMA)
Zhou, Jun& Liang, Tian& Wang, Dejun& Li, Liru& Cheng, Yan& Guo, Qiuyan…[et al.]. IFNα-Expressing Amniotic Fluid-Derived Mesenchymal Stem Cells Migrate to and Suppress HeLa Cell-Derived Tumors in a Mouse Model. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213120
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213120