Type III Transforming Growth Factor-β Receptor RNA Interference Enhances Transforming Growth Factor β3-Induced Chondrogenesis Signaling in Human Mesenchymal Stem Cells
Joint Authors
Zheng, Shuhui
Lian, Chengjie
Zhou, Taifeng
Gao, Bo
Xu, Caixia
Su, Peiqiang
Zhou, Hang
Chen, Zhuohui
Li, Yongyong
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-08
Country of Publication
Egypt
No. of Pages
11
Abstract EN
The type III transforming growth factor-β (TGF-β) receptor (TβRIII), a coreceptor of the TGF-β superfamily, is known to bind TGF-βs and regulate TGF-β signaling.
However, the regulatory roles of TβRIII in TGF-β-induced mesenchymal stem cell (MSC) chondrogenesis have not been explored.
The present study examined the effect of TβRIII RNA interference (RNAi) on TGF-β3-induced human MSC (hMSC) chondrogenesis and possible signal mechanisms.
A lentiviral expression vector containing TβRIII small interfering RNA (siRNA) (SiTβRIII) or a control siRNA (SiNC) gene was constructed and infected into hMSCs.
The cells were cultured in chondrogenic medium containing TGF-β3 or control medium.
TβRIII RNAi significantly enhanced TGF-β3-induced chondrogenic differentiation of hMSCs, the ratio of type II (TβRII) to type I (TβRI) TGF-β receptors, and phosphorylation levels of Smad2/3 as compared with cells infected with SiNC.
An inhibitor of the TGF-β signal, SB431542, not only inhibited TβRIII RNAi-stimulated TGF-β3-mediated Smad2/3 phosphorylation but also inhibited the effects of TβRIII RNAi on TGF-β3-induced chondrogenic differentiation.
These results demonstrate that TβRIII RNAi enhances TGF-β3-induced chondrogenic differentiation in hMSCs by activating TGF-β/Smad2/3 signaling.
The finding points to the possibility of modifying MSCs by TβRIII knockdown as a potent future strategy for cell-based cartilage tissue engineering.
American Psychological Association (APA)
Zheng, Shuhui& Zhou, Hang& Chen, Zhuohui& Li, Yongyong& Zhou, Taifeng& Lian, Chengjie…[et al.]. 2018. Type III Transforming Growth Factor-β Receptor RNA Interference Enhances Transforming Growth Factor β3-Induced Chondrogenesis Signaling in Human Mesenchymal Stem Cells. Stem Cells International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1213387
Modern Language Association (MLA)
Zheng, Shuhui…[et al.]. Type III Transforming Growth Factor-β Receptor RNA Interference Enhances Transforming Growth Factor β3-Induced Chondrogenesis Signaling in Human Mesenchymal Stem Cells. Stem Cells International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1213387
American Medical Association (AMA)
Zheng, Shuhui& Zhou, Hang& Chen, Zhuohui& Li, Yongyong& Zhou, Taifeng& Lian, Chengjie…[et al.]. Type III Transforming Growth Factor-β Receptor RNA Interference Enhances Transforming Growth Factor β3-Induced Chondrogenesis Signaling in Human Mesenchymal Stem Cells. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1213387
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213387