Vitamin D Promotes MSC Osteogenic Differentiation Stimulating Cell Adhesion and αVβ3 Expression
Joint Authors
Mori, Giorgio
Di Benedetto, Adriana
Porro, Chiara
Trotta, Teresa
Posa, Francesca
Cavalcanti-Adam, Elisabetta A.
Brunetti, Giacomina
Grano, Maria
Colaianni, Graziana
Lo Muzio, Lorenzo
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-28
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Vitamin D (Vit D) by means of its biological active form, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3), has a protective effect on the skeleton by acting on calcium homeostasis and bone formation.
Furthermore, Vit D has a direct effect on mesenchymal stem cells (MSCs) in stimulating their osteogenic differentiation.
In this work, we present for the first time the effect of 1,25(OH)2D3 on MSC adhesion.
Considering that cell adhesion to the substrate is fundamental for cell commitment and differentiation, we focused on the expression of αVβ3 integrin, which has a key role in the commitment of MSCs to the osteoblastic lineage.
Our data indicate that Vit D increases αVβ3 integrin expression inducing the formation of focal adhesions (FAs).
Moreover, we assayed MSC commitment in the presence of the extracellular matrix (ECM) glycoprotein fibronectin (FN), which is able to favor cell adhesion on surfaces and also to induce osteopontin (OPN) expression: this suggests that Vit D and FN synergize in supporting cell adhesion.
Taken together, our findings provide evidence that Vit D can promote osteogenic differentiation of MSCs through the modulation of αVβ3 integrin expression and its subcellular organization, thus favoring binding with the matrix protein (FN).
American Psychological Association (APA)
Posa, Francesca& Di Benedetto, Adriana& Cavalcanti-Adam, Elisabetta A.& Colaianni, Graziana& Porro, Chiara& Trotta, Teresa…[et al.]. 2018. Vitamin D Promotes MSC Osteogenic Differentiation Stimulating Cell Adhesion and αVβ3 Expression. Stem Cells International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1213533
Modern Language Association (MLA)
Posa, Francesca…[et al.]. Vitamin D Promotes MSC Osteogenic Differentiation Stimulating Cell Adhesion and αVβ3 Expression. Stem Cells International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1213533
American Medical Association (AMA)
Posa, Francesca& Di Benedetto, Adriana& Cavalcanti-Adam, Elisabetta A.& Colaianni, Graziana& Porro, Chiara& Trotta, Teresa…[et al.]. Vitamin D Promotes MSC Osteogenic Differentiation Stimulating Cell Adhesion and αVβ3 Expression. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1213533
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213533