Isolation and Molecular Identification and Antimicrobial Susceptibility of Providencia spp. from Raw Cow’s Milk in Baghdad, Iraq

Author

Ayyal Al‐Gburi, Nagham Mohammed

Source

Veterinary Medicine International

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-11-19

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Zoology

Abstract EN

A total of sixty raw milk samples were collected from (street vendors and shops) from Baghdad city, Iraq.

The samples were inoculated into peptone water and, then, subcultured onto MacConkey agar and Blood agar.

Identification of isolates was confirmed by microscopic examination, cultural characteristic, biochemical tests, Vitek (VITEK®2 system), and Biolog GN substrate reactions followed by 16S rRNA and specific genes sequencing.

Of 60 raw cow’s milk samples, Providencia spp.

were identified only in 4 samples (6.67%) and P.

rettgeri was the most common, 2/4 (50%), followed by P.

stuartii and P.

vermicola, 1/4 (25%).

Antimicrobial susceptibility tests were conducted against ten antibiotics by the disc diffusion method.

All Providencia isolates showed multidrug resistance (MDR), and the absolute resistant was 100% to tetracycline, erythromycin, and doxycycline and 50% against ampicillin\sulbactam and amoxicillin/clavulanic acid.

They were highly susceptible (100%) to trimethoprim, imipenem, and chloramphenicol.

These findings indicate that milk might be contaminated with Providencia spp.

leading to transmission to humans causing poisoning, diarrhea, and other infections.

This is the first study of isolated Providencia spp.

from raw cow’s milk.

American Psychological Association (APA)

Ayyal Al‐Gburi, Nagham Mohammed. 2020. Isolation and Molecular Identification and Antimicrobial Susceptibility of Providencia spp. from Raw Cow’s Milk in Baghdad, Iraq. Veterinary Medicine International،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1214273

Modern Language Association (MLA)

Ayyal Al‐Gburi, Nagham Mohammed. Isolation and Molecular Identification and Antimicrobial Susceptibility of Providencia spp. from Raw Cow’s Milk in Baghdad, Iraq. Veterinary Medicine International No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1214273

American Medical Association (AMA)

Ayyal Al‐Gburi, Nagham Mohammed. Isolation and Molecular Identification and Antimicrobial Susceptibility of Providencia spp. from Raw Cow’s Milk in Baghdad, Iraq. Veterinary Medicine International. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1214273

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1214273