عزل و تكثير الخلايا الجذعية الميزنشيمية من نسيج الحبل السري باستعمال مصل دم الحبل السري كبديل للمصل الجنيني البقري

Abstract EN

Mesenchymal stem cells are multipotent stem cells that can be isolated from umbilical cord and can be used therapeutically because of their ability to differentiate to various types of cells, in addition to their immunosuppressive and anti-inflammatory characteristics.

We here employed simple and economically efficient protocol to isolate these cells without using enzymes to digest umbilical cord tissues and replaced fetal bovine serum with umbilical cord blood serum 10% and studed the effect of this replacement on morphology and cell stemness characteristicsin comparion with fetal bovine serum 20%.

We found that umbilical cord blood serum could be used to isolate stem cells from the umbilical cord in a simple manner by cutting umbilical cord and transplant the pieces immediately.

In addition, umbilical cord blood serum 10% could be used instead of fetal bovine serum 20% for cell isolation and expansion, in which the stem cells maintained their stemness characters (ability to differentiate to adipocytes and osteocytes and express mesenchymal stem cell specific clusters of differentiation (CD44, CD90, CD105).