Enhancing tomato plant resistance against tobacco mosaic virus using riboflavin

Other Title(s)

حث مقاومة نباتات الطماطم ضد فيروس تبرقش الدخان باستخدام الريبوفلافين

Joint Authors

Mahmud, Faridah Muhammad
Bunduq, Ahmad Muhsin
Khalifah, Wala
Abu al-Abbas, Fawzi

Source

Arab Universities Journal of Agricultural Sciences

Issue

Vol. 28, Issue 4 (31 Dec. 2020), pp.1257-1270, 14 p.

Publisher

Ain Shams University Faculty of Agriculture

Publication Date

2020-12-31

Country of Publication

Egypt

No. of Pages

14

Main Subjects

Agriculture

Topics

Abstract EN

Plant systemic resistance induced by natural product is an alternative technique of disease management.

Riboflavin (vitamin B2) usually used as abiotic elicitor to improve the plant immunity against different pathogens.

This work aimed to explore the efficiency of three riboflavin concentrations (0.5, 2.5, and 5 mM) to enhance resistance toward Tobacco Mosaic Virus (TMV) in tomato plants.

Our results showed that exogenous application of 2.5mM riboflavin, 5 days before virus challenge was the most effective concentration, which provided a reduction in disease incidence and disease severity by 80% and 75% , respectively.

Furthermore, obtained results were confirmed by using DAS-ELISA test, which showed that concentration 2.5 mM of riboflavin decreased the virus concentrations by 46.4% in treated- inoculated plants.

It was remarked that exogenous application of 2.5 mM riboflavin showed a positive effect on some plant growth parameters either in presence or ence of TMV challenge.

The plant height and number of leaves per plant were significantly improved in treated- inoculated plants about 30% and in compared to the infected control 78% .

In time course investigation, 2.5mM riboflavin treatment reduced the virus symptoms particularly at 9th day, where after the symptoms become evident.

In addition, expression of phenylalanine ammonia-lyase (PAL) and pathogenesis-related protein (PR10), which are the markers of systemic acquired resistance (SAR), were rapidly increased in inoculated-treated tomato from 1-3 days after treatment.

Moreover, the results of the biochemical changes analysis revealed that, the levels of the defense markers including peroxidase (PO), and polyphenol oxidase (PPO) were increased four days after of treatment and reached maximum levels at 8 days in the treated- inoculated plants.

In conclusion, it could be suggesting that riboflavin exerted a great influence on TMV disease, as indicated by reduction of disease symptoms as well as enhancement of biochemical changes in plant defense against infection with TMV.

American Psychological Association (APA)

Mahmud, Faridah Muhammad& Bunduq, Ahmad Muhsin& Khalifah, Wala& Abu al-Abbas, Fawzi. 2020. Enhancing tomato plant resistance against tobacco mosaic virus using riboflavin. Arab Universities Journal of Agricultural Sciences،Vol. 28, no. 4, pp.1257-1270.
https://search.emarefa.net/detail/BIM-1240553

Modern Language Association (MLA)

Mahmud, Faridah Muhammad…[et al.]. Enhancing tomato plant resistance against tobacco mosaic virus using riboflavin. Arab Universities Journal of Agricultural Sciences Vol. 28, no. 4 (2020), pp.1257-1270.
https://search.emarefa.net/detail/BIM-1240553

American Medical Association (AMA)

Mahmud, Faridah Muhammad& Bunduq, Ahmad Muhsin& Khalifah, Wala& Abu al-Abbas, Fawzi. Enhancing tomato plant resistance against tobacco mosaic virus using riboflavin. Arab Universities Journal of Agricultural Sciences. 2020. Vol. 28, no. 4, pp.1257-1270.
https://search.emarefa.net/detail/BIM-1240553

Data Type

Journal Articles

Language

English

Notes

-

Record ID

BIM-1240553