The protective effect of alpha-lipoic acid on the expression of collagen iv, renal function, and oxidative stress induced by diazinon in the renal parenchyma of rat
Joint Authors
Delavar, Amir
Valokola, Mahmud Gorji
Anbarkeh, Fatimah Rahim
Nikravesh, Muhammad Rida
Jalali, Mahdi
Source
Saudi Journal of Kidney Diseases and Transplantation
Issue
Vol. 31, Issue 6 (31 Dec. 2020), pp.1310-1319, 10 p.
Publisher
Saudi Center for Organ Transplantation
Publication Date
2020-12-31
Country of Publication
Saudi Arabia
No. of Pages
10
Main Subjects
Abstract EN
Diazinon (DZN) is an organophosphate pesticide that is commonly used in agriculture worldwide, including in Iran, and unfortunately, it leads to a variety of negative effects on the environment, animals, and humans.
Alpha-lipoic acid (ALA) is an antioxidant agent that acts via scavenging of oxygen-free radicals.
Collagen IV is a component of the main base membrane structure and DZN may also affect the expression of this key protein.
The aim of this study was to evaluate antioxidant properties of ALA on the expression of collagen IV, renal function, and oxidative stress induced by DZN in renal tissue.
In this experimental study, 30 adult male Wistar rats were randomly divided into five groups (n = 6) including: the control group, DZN (40 mg/kg) group, ALA (100 mg/kg) group, ALA (100 mg/kg) + DZN (40 mg/kg) group, and sham group.
On day 0 and after 6 weeks, the urine and blood samples were collected to measure glomerular filtration rate (GFR).
After 6 weeks, the rats were anesthetized and the left kidney was used for immunohistochemistry study and the right kidney was used to evaluate the oxidative stress parameters.
The results have shown that ALA significantly improved the biochemical parameters including superoxide dismutase, glutathione peroxidase, glutathione S- transferase, glutathione reductase, and GFR.
In addition, ALA significantly prevented the expression of collagen IV that was changed by DZN administration in rats.
We concluded that when exposed to DZN, depletion of antioxidant enzymes is accompanied by the induction of oxidative stress that might be beneficial in monitoring DZN toxicity and alpha-lipoic acid, as an antioxidant can overcome the toxicity induced by DZN in the kidney.
American Psychological Association (APA)
Delavar, Amir& Nikravesh, Muhammad Rida& Jalali, Mahdi& Valokola, Mahmud Gorji& Anbarkeh, Fatimah Rahim. 2020. The protective effect of alpha-lipoic acid on the expression of collagen iv, renal function, and oxidative stress induced by diazinon in the renal parenchyma of rat. Saudi Journal of Kidney Diseases and Transplantation،Vol. 31, no. 6, pp.1310-1319.
https://search.emarefa.net/detail/BIM-1242348
Modern Language Association (MLA)
Delavar, Amir…[et al.]. The protective effect of alpha-lipoic acid on the expression of collagen iv, renal function, and oxidative stress induced by diazinon in the renal parenchyma of rat. Saudi Journal of Kidney Diseases and Transplantation Vol. 31, no. 6 (Nov. / Dec. 2020), pp.1310-1319.
https://search.emarefa.net/detail/BIM-1242348
American Medical Association (AMA)
Delavar, Amir& Nikravesh, Muhammad Rida& Jalali, Mahdi& Valokola, Mahmud Gorji& Anbarkeh, Fatimah Rahim. The protective effect of alpha-lipoic acid on the expression of collagen iv, renal function, and oxidative stress induced by diazinon in the renal parenchyma of rat. Saudi Journal of Kidney Diseases and Transplantation. 2020. Vol. 31, no. 6, pp.1310-1319.
https://search.emarefa.net/detail/BIM-1242348
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1317-1419
Record ID
BIM-1242348