How reliable are general histopathologists in detection of helicobacter pylori on routine histology ?

Author

Abd Allah, Saman S.

Source

Medical Journal of Babylon

Issue

Vol. 18, Issue 2 (30 Jun. 2021), pp.83-88, 6 p.

Publisher

University of Babylon College of Medicine

Publication Date

2021-06-30

Country of Publication

Iraq

No. of Pages

6

Main Topic

Biology

Topics

Abstract EN

Background: Many studies agree that routine histology is less sensitive and less specific than special stains and immunohistochemistry (IHC) with false‑positive and false‑negative rates.

Objectives: The aim was to evaluate the accuracy of four general histopathologists in documenting Helicobacter pylori (Hp) bacteria in gastric biopsies (stained with H and E) by using IHC and modified Giemsa stain (MG), also to assess the specificity of MG and whether it can replace IHC.

Materials and Methods: Twenty seven reported gastric biopsies were collected from each of four pathologists (9 reported as negative for Hp, 9 reported as scant Hp, and 9 as abundant Hp).

The pathologists selected were known to have different thresholds for Hp.

The biopsies were stained with MG and IHC and they were read by two pathologists.

Results: The four pathologists showed different thresholds for labeling luminal gastric pits' structures as Hp.

Overall, there were 5/36 false‑negative cases(13.9% ) and 26/72 false‑positive cases (36% ), most of them in the category reported as scant Hp detected.

The results of MG were concordant with that of IHC in 107/108 cases.

Conclusion: The overall accuracy rate of Hp detection on routine histology was 71% ; false positivity was much more common than false negativity, especially when only scant Hp reported to be present.

MG is a reliable stain and if done properly, it can replace the more costly IHC.

When Hp identification is crucial from the clinical management of view, special stains and/or IHC should be Background: Many studies agree that routine histology is less sensitive and less specific than special stains and immunohistochemistry (IHC) with false‑positive and false‑negative rates.

Objectives: The aim was to evaluate the accuracy of four general histopathologists in documenting Helicobacter pylori (Hp) bacteria in gastric biopsies (stained with H and E) by using IHC and modified Giemsa stain (MG), also to assess the specificity of MG and whether it can replace IHC.

Materials and Methods: Twenty seven reported gastric biopsies were collected from each of four pathologists (9 reported as negative for Hp, 9 reported as scant Hp, and 9 as abundant Hp).

The pathologists selected were known to have different thresholds for Hp.

The biopsies were stained with MG and IHC and they were read by two pathologists.

Results: The four pathologists showed different thresholds for labeling luminal gastric pits' structures as Hp.

Overall, there were 5/36 false‑negative cases(13.9% ) and 26/72 false‑positive cases (36% ), most of them in the category reported as scant Hp detected.

The results of MG were concordant with that of IHC in 107/108 cases.

Conclusion: The overall accuracy rate of Hp detection on routine histology was 71% ; false positivity was much more common than false negativity, especially when only scant Hp reported to be present.

MG is a reliable stain and if done properly, it can replace the more costly IHC.

When Hp identification is crucial from the clinical management of view, special stains and/or IHC should be requested.

American Psychological Association (APA)

Abd Allah, Saman S.. 2021. How reliable are general histopathologists in detection of helicobacter pylori on routine histology ?. Medical Journal of Babylon،Vol. 18, no. 2, pp.83-88.
https://search.emarefa.net/detail/BIM-1248079

Modern Language Association (MLA)

Abd Allah, Saman S.. How reliable are general histopathologists in detection of helicobacter pylori on routine histology ?. Medical Journal of Babylon Vol. 18, no. 2 (Apr. / Jun. 2021), pp.83-88.
https://search.emarefa.net/detail/BIM-1248079

American Medical Association (AMA)

Abd Allah, Saman S.. How reliable are general histopathologists in detection of helicobacter pylori on routine histology ?. Medical Journal of Babylon. 2021. Vol. 18, no. 2, pp.83-88.
https://search.emarefa.net/detail/BIM-1248079

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 87-88

Record ID

BIM-1248079