Multi antibiogram-integron relationship in salmonella species isolated from local poultry carcasses \

Abstract EN

This investigation was carried out to investigate the relationship between incidence of multidrug-resistant isolates and presence of class 1 integron in Salmonella recovered from poultry carcasses from local Egyptian markets.

One hundred samples of raw chicken carcasses which obtained from ten poultry markets at Alexandria markets were examined for presence of Salmonella sp., via conventional microbiological methods, serotyping, real time PCR and lastly Conventional PCR for screening of Salmonella for sequencing and Class-1 Integrons presence.

Detection rate of Salmonella sp.

was (6%) with serotypes S.

kentucky, S.

typhimurium, S.

minnesota and S.

arizonae with positive results for Salmonella for conventional and quantitative PCR.

Based on sequenced specific amplicons with 204 bp, Salmonella isolate no.1 was more identical to isolate no.2 which showed highest similarity to isolate no.4, while Isolate no.3 was more identical to isolate no.6, Isolate no.4 was more identical to isolate no.2, Isolate no.5 was more identical to isolate no.4.

Lastly, isolate no.6 was more identical to isolate no.3.

comparing with gene bank database, first, second, third, fourth, fifth and sixth isolates identified as S.

kentucky, S.

minnesota, S.

kentucky, S.

minnesota and S.

typhimurium and S.

arizonae respectively.

Obtained results indicated that all six isolates considered multi-resistance isolates as (100 %) with varied resistance patterns.

Class-1 Integron detection results reflected that first, second and fourth samples were Positive for class 1 integron (750 bp) which indicated relationship between antibiotic resistance and presence of integron in bacteria.

Our obtaining findings cleared correlation between high antibiotics resistance rates exhibited by Salmonella isolates and presence of class 1 integron gene cassettes harboring resistance genes.