تقييم الثبات الجيني لأنسجة نخيل التمر (Phoenix Dactylifera L.)‎ صنف الحلاوي المكثرة خارج الجسم الحي باستخدام الواسمات الجزيئية RAPD

Abstract EN

The study was conducted in the laboratories of Date Palm Research Center to study some plant growth regulators in the induction of callus in the date palm and its effect on causing genetic changes at the level of callus.

This experiment carried out; four treatments were used that included (1) the control (free of growth regulators) and three treatments that gave a response to callus induction; they were the treatment (2) that included 2, 4-D (5 mg.

l-1) + NAA (5 mg.

l-1) ) +2i P (1 mg.

l-1) and treatment (3) including 2, 4-D 5) mg.

l-1)+1) NAA mg.

l-1) + BA (1 mg.

l-1) and treatment (4) including, 4-D) 2 5 mg.

l-1) + 5) NAA mg.

l-1) + NOA (5 mg.

l-1) + IAA (1 mg.

l-1) + IBA (1 mg.

l-1) + 2i P (3 mg.

l-1) + BA) 3 mg.

l-1).

The response rate for callus induction varied, reaching its maximum rate in treatment (2) recorded (66.4% ), while control treatment (1) recorded (4.8% ) as the lowest rate in callus induction.

Also, treatment (2) recorded the highest fresh and dry weight of (0.894) and (0.0844) g with a significant increase compared with other treatments, while the control recorded the lowest value of (0.228 and 0.0127) g of fresh and dry weight.

The results showed that there was a genetic match for the callus resulting from the treatments between the seedling and the mother plant, except for the first treatment (control) free of growth regulators, which gave one band different from the seedling and the mother plant, and the fourth treatment, which contained some auxins and cytokinins, which gave two clear bands showing the emergence of genetic variation as a result of hormonal imbalance in cells of explant.