Molecular and serological detection of toxoplasma gondii in random sample of pregnant and aborted women of Nineveh city in Iraq

Abstract EN

The present study is to identify the rate of parasitic disease of toxoplasmosis among among females from 120 pregnant and aborted women attending to the Department of Obstetrics and Gynecology of the Al-Kansaa, AL-Salaam Hospital and 2 private clinics during (September 2019-febrauary 2020).

Three methods of tests to search for toxoplasmosis latex test, combo IgG and IgM rapid cassette and finally by PCR.

Questionnaire is applying by pregnant and aborted patients and according to the information the females was divided to groups involved age, career, gestational period, housing cat or not in living zone, number of abortion,blood group and according to residence.

The results show big rate of toxoplasmosis by latex test the positive cases 62 (62 %).the result re-tested by another serological method combo IgG and IgM rapid cassette and give the result 44 (73.33 %) and 6 (9.09 %) for IgG and IgM respectively.

The result was confirmed by third test using molecular test conventional PCR to show the result 32 (51.61 %).

We used EDTA tube for whole blood for PCR, gel tube for serological testes.

Three methods of tests to search for toxoplasmosis latex test, combo IgG and IgM rapid cassette and finally by PCR.