نسب الخلايا التائية المساعدة (Th2 و Th1 وTh17)‎ في الدوران لدى مريضات سرطان الثدي و مريضات الأورام الحميدة في الثدي

Abstract EN

Objective: Dysfunction of systemic immunity may be involved in the establishment of advanced breast cancer.

Therefore, it is important to understand immune-regulation via estimation of T helper (Th1, Th2 and Th17) cell frequencies in peripheral blood of breast cancer patients.

Methods: Sixty nine patients with breast tumors were enrolled in this study.

Based on pathologic diagnostic assessment, patients were divided into two categories: patients affected with benign breast tumors (n=28) with age range (26-79) years, and breast cancer patients (n=41) with age range (22-74) years.

This study investigated the distribution of Th1 (CD4+IFNy+), Th2 (CD4+IL-4+) and Th17 (CD4+IL-17+) in peripheral blood mononuclear cells (PBMCs) from two studied groups.

Cells were evaluated by flow cytometry and reported as a percentage of total CD4+ cells.

The present study also assessed the association of cell percentages with clinicopathological parameters.

Results: Compared with patients of benign breast tumors, an increase in the peripheral percentages of Th1 and Th2 was observed in breast cancer patients, while the percentage of Th17 cells increased.

However; no significant difference of cell percentages was found between the two studied groups.

Elevation of Th2 frequency in breast cancer patients correlated with lymph node metastasis.

However; no significant association was recognized between the frequencies of other cell subtypes and clinicopathological factors.

Conclusions: We observed significantly increased percentage of circulating Th2 cells in breast cancer patients with metastasis.

These results indicate that Th2 may play an important role in metastasis of breast malignancy.