Molecular screening of some β-lactam resistance genes producing clinical isolates of acinetobacter baumannii

Abstract EN

Acinetobacter baumannii is currently as a main opportunistic bacteria to human that posing an rising risk to persons admitted to the hospital, causing them satisfactory infection.

Subject of this work aimed to study the dissemination and the frequency of some plasmid genes that encoding Extended Spectrum β-Lactamase (ESBL) and Metallo β-Lactamase (MBL) among A.

baumannii isolates.

A total of 235 of various samples (burns, urine and blood) from hospitalized patients in Al-Hussain General Hospital during a period of five months between June to October 2014.

The isolates were identified using microscopic examination, all positive bacterial samples were submitted to conventional biochemical tests and the suspected isolates were candidate to detect 16-23S rRNA using PCR and finally was diagnosed bacteria to confirm the species level, depending on the results of Vitek 2 System.

The results were revealed that only 9 isolates identify as A.

baumannii and 1 isolate as A.

baumannii complex.

The results revealed high resistance to different groups of antibiotics, all isolates were resistance 100% to Ampicillin, cefoxitin, ceftazidime, cefotaxime, cefepime and aztreonam, while it was 70% to ciprofloxacin and 60% to gentamicin and amikacin while 50% of isolates were resistant to imipenem.

Molecular screening of tested antimicrobial resistance genes were performed, results revealed that different percent of occurrence as following: bla TEM-1(100%), bla TEM-2(90%), bla OXA-1(90%), bla OXA-2(20%), bla OXA-10(30%), and bla IMP(10%).

While bla (OXA-23, OXA-48 and KPC ) genes were no detected in this work.

Conclusion.

The spread of β-lactam resistance genes especially ESBLs that produced by A.

baumannii which detected in this study is a large worry therefore, must take the necessary measures and procedures to reduce this prevalence in the hospitals.