Detection and quantitative estimation of toxic acrylamide levels in selected potatoes chips and French fries from the Libyan market using HPLC-UV method
Other Title(s)
الكشف والتقدير الكمي لمستوى مادة الأكريلاميد السامة في رقائق البطاطس، و البطاطس المقلية المختارة من السوق الليبي باستخدام طريقة HPLC-UV
Joint Authors
Kharit, Usamah Idris G.
al-Fuwiris, Abd al-Salam Fadil
al-Jali, Abd al-Rahman A.
Abd al-Jalil, Um Kulthum Ahmad
Source
Al-Mukhtar Journal of Sciences
Issue
Vol. 36, Issue 2 (30 Jun. 2021), pp.106-115, 10 p.
Publisher
Publication Date
2021-06-30
Country of Publication
Libya
No. of Pages
10
Main Subjects
Abstract EN
Acrylamide is a potential health hazardous compound occurring in baked and fried food as a result of excessive dry heating during the preparation and/or processing of foods.
Exposure to a high level of acrylamide may cause cancer, neurotoxicity, and mutagenicity.
In this study, an isocratic reversed-phase high-performance liquid chromatographic (HPLC) method using a C18 column was used for the determination of acrylamide in selected food.
The mobile phase consisted of 0.1% formic acid in water: acetonitrile (98:02), and the flow rate was 1.0 mL min-1, elution was monitored at 200 nm.
Validation in selected conditions showed that the chosen method is sensitive, selective, precise, and reproducible with a linear detector response for the determination of acrylamide.
The limit of detection (LOD), and the limit of quantification (LOQ), were achieved at 0.41μg mL-1 and 1.25 μg mL-1respectively.
The proposed method was also applied after validation to the most popular six brands of chips and French fries available in the Libyan market.
Acrylamide was extracted by a simplified extraction method avoiding cleanup by solid-phase extraction (SPE), then analyzed by HPLC-UV.
The highest level of acrylamide was found in one brand of chips with a concentration of 16.33 μg mL-1, whereas only one of the French fries products analyzed exhibited an acrylamide concentration of 10.26 μg mL-1.
American Psychological Association (APA)
Kharit, Usamah Idris G.& al-Fuwiris, Abd al-Salam Fadil& al-Jali, Abd al-Rahman A.& Abd al-Jalil, Um Kulthum Ahmad. 2021. Detection and quantitative estimation of toxic acrylamide levels in selected potatoes chips and French fries from the Libyan market using HPLC-UV method. Al-Mukhtar Journal of Sciences،Vol. 36, no. 2, pp.106-115.
https://search.emarefa.net/detail/BIM-1324429
Modern Language Association (MLA)
Kharit, Usamah Idris G.…[et al.]. Detection and quantitative estimation of toxic acrylamide levels in selected potatoes chips and French fries from the Libyan market using HPLC-UV method. Al-Mukhtar Journal of Sciences Vol. 36, no. 2 (2021), pp.106-115.
https://search.emarefa.net/detail/BIM-1324429
American Medical Association (AMA)
Kharit, Usamah Idris G.& al-Fuwiris, Abd al-Salam Fadil& al-Jali, Abd al-Rahman A.& Abd al-Jalil, Um Kulthum Ahmad. Detection and quantitative estimation of toxic acrylamide levels in selected potatoes chips and French fries from the Libyan market using HPLC-UV method. Al-Mukhtar Journal of Sciences. 2021. Vol. 36, no. 2, pp.106-115.
https://search.emarefa.net/detail/BIM-1324429
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 111-114
Record ID
BIM-1324429