A biochemical study of α-amylase activity in saliva of some Libyan cigarette smokers
Other Title(s)
دراسة بيوكيماوية لنشاط أنزيم ألفا أميليز في لعاب بعض المدخنين الليبيين
Joint Authors
al-Salhin, Khalid S.
Umar, Um al-Sad E. I.
al-Jarmi, Latifah Ahmad
Source
Al-Mukhtar Journal of Sciences
Issue
Vol. 35, Issue 3 (30 Sep. 2020), pp.195-204, 10 p.
Publisher
Publication Date
2020-09-30
Country of Publication
Libya
No. of Pages
10
Main Subjects
Abstract EN
Tobacco consumption alters many biological parameters, including α-amylase activ- ity.
This study reports the effect of cigarette smoking on salivary α-amylase activities of some male heavy smokers (100) in apparent good health compared with (40) male non-smokers as control.
The salivary α-amylase activity was assayed using the standard colorimetric method.
The α-amylase enzyme was characterized from the saliva samples collected from healthy adult smokers and non-smokers (control) to determine the effects of temperature, pH, and substrate concentration on the kinetic parameters of the enzyme.
The analysis of the saliva samples showed that there were significant increases (P<0.05) in the α-amylase activity values in the saliva of cigarette smokers when compared with non-smokers at optimum conditions (2.74±0.37 nmol/min/mg-protein and 2.01±0.16 nmol/min/mg-protein, respectively).
The Michaelis constant (Km) values of 0.5±0.02 mM and 1.12±0.08 mM observed for non-smokers and smokers were obtained from the double reciprocal plot of initial velocity (1/V) and sub- strate concentration (1/[S]).
The maximum activities obtained at an optimum temperature of 37oC, and an apparent pH of 7.0 for both subjects were 31.25±8.24 nmol/min/mg protein and 18.10±4.06 nmol/min/mg protein for non-smokers and smokers respectively.
Measurements of salivary α-amylase activity could be useful in the early detection and diagnosis of parotitis.
Subsequent studies are required to specify the mechanisms responsible for such a risk.
American Psychological Association (APA)
al-Salhin, Khalid S.& Umar, Um al-Sad E. I.& al-Jarmi, Latifah Ahmad. 2020. A biochemical study of α-amylase activity in saliva of some Libyan cigarette smokers. Al-Mukhtar Journal of Sciences،Vol. 35, no. 3, pp.195-204.
https://search.emarefa.net/detail/BIM-1324837
Modern Language Association (MLA)
al-Salhin, Khalid S.…[et al.]. A biochemical study of α-amylase activity in saliva of some Libyan cigarette smokers. Al-Mukhtar Journal of Sciences Vol. 35, no. 3 (2020), pp.195-204.
https://search.emarefa.net/detail/BIM-1324837
American Medical Association (AMA)
al-Salhin, Khalid S.& Umar, Um al-Sad E. I.& al-Jarmi, Latifah Ahmad. A biochemical study of α-amylase activity in saliva of some Libyan cigarette smokers. Al-Mukhtar Journal of Sciences. 2020. Vol. 35, no. 3, pp.195-204.
https://search.emarefa.net/detail/BIM-1324837
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 201-203
Record ID
BIM-1324837