Molecular characterization of some virulence genes and antibiotic susceptibility pattern among Uropathogenic Escherichia coli Isolated from Patient in Zakho City Iraq
Joint Authors
Ibrahim, Marwah S.
Khalid, Haval M.
Mero, Wijdan M. S.
Source
ZANCO Journal of Pure and Applied Sciences
Issue
Vol. 32, Issue 2 (30 Apr. 2020), pp.167-177, 11 p.
Publisher
Salahaddin University-Erbil Department of Scientific Publications
Publication Date
2020-04-30
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Pharmacy, Health & Medical Sciences
Abstract EN
Uropathogenic Escherichia coli (UPEC) is one of the most causative agents which causing urinary tract infections (UTIs) in humans.
This study involved the prevalence of the virulence genes among UPEC isolated from patients in various hospitals in Zakho city from July 2018 until January 2019 and their susceptibility to different commonly used antimicrobial agent against UPEC.
The different culture media were used for the identification of Escherichia coli (E.
coli).
Out of 400 samples, 141 (35.25%) strains of UPEC were isolated from enrolled patients.
The antibiotic susceptibility toward different antibiotics was varied among the isolates.
Imipenem was the most potent antibiotic with a resistant rate of only 2.84%.
While the isolates were resistant to most screened antibiotics, with the highest rate 96.45% to Amoxicillin/ clavulanic.
The resistant rates decreased toward other antibiotics at rates varied from 93.62% for Amoxicillin to 43.97% for Norfloxacin.
Fifty-seven isolates were selected for PCR analysis, according to the resistance of E.coli to various antibiotics.
The selected samples were successfully amplified for E.
coli identification by producing a single band of a target uidA gene.
In this study, the virulence related genes were detected in only 35 (61.40%) isolates out of 57 isolates.
The distribution of the virulence related genes that included; afa, sfa, hly, cnf and pai were 28.07%, 17.54%, 26.32%, 22.81% and 22.81%, respectively.
The study highlight that multidrug resistance UPEC harbors multiple virulence genes circulating in this setting.
American Psychological Association (APA)
Ibrahim, Marwah S.& Khalid, Haval M.& Mero, Wijdan M. S.. 2020. Molecular characterization of some virulence genes and antibiotic susceptibility pattern among Uropathogenic Escherichia coli Isolated from Patient in Zakho City Iraq. ZANCO Journal of Pure and Applied Sciences،Vol. 32, no. 2, pp.167-177.
https://search.emarefa.net/detail/BIM-1386632
Modern Language Association (MLA)
Ibrahim, Marwah S.…[et al.]. Molecular characterization of some virulence genes and antibiotic susceptibility pattern among Uropathogenic Escherichia coli Isolated from Patient in Zakho City Iraq. ZANCO Journal of Pure and Applied Sciences Vol. 32, no. 2 (2020), pp.167-177.
https://search.emarefa.net/detail/BIM-1386632
American Medical Association (AMA)
Ibrahim, Marwah S.& Khalid, Haval M.& Mero, Wijdan M. S.. Molecular characterization of some virulence genes and antibiotic susceptibility pattern among Uropathogenic Escherichia coli Isolated from Patient in Zakho City Iraq. ZANCO Journal of Pure and Applied Sciences. 2020. Vol. 32, no. 2, pp.167-177.
https://search.emarefa.net/detail/BIM-1386632
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references.
Record ID
BIM-1386632