Molecular diagnosis of fasciola hepatica in livestock using cox1 gene in Erbil provence-Kurdistan region Iraq

Abstract EN

52 Fasciola hepatica worms (22 sheep, 3 goats and 27 cattle) from livers and bile ducts were collected.

The prevalence rate of Fascioliasis was 7.28%, 3.37% and 10.19%, respectively.

Among the three hosts regarding the sex, females of sheep and goats show a higher infection rate than males (4.3% and 2.25% versus 2.98% and 1.12%).

Simultaneously, the prevalence rate was slightly higher in female cattle (5.28%) than male cattle (4.91%).

Used the adult worms for DNA extraction followed by PCR amplification.

The sequences of amplified products were identified under accession number (MT951586) of sheep were 99.51%, and (MT951587) of goat was 99.75% homologous to Iranian goat Fasciola hepatica (KF992219).

Furthermore, the cattle Fasciola hepatica (MT951585) was 99.2% homologous with the reference of Iranian goat.

Using phylogenetic tree analysis, isolated F.

hepatica in sheep, goats, and cattle is positively related to various global F.

hepatica.